FLUORESCENCE SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS IN THE HONEY SAMPLES CLASSIFICATION

Abstract

Steady state fluorescence spectroscopy in combination with Principal Component Analysis (PCA) for spectral analysis was used to differentiate multifloral honeys from different parts of Serbia. The emission spectra were recorded in the wavelength range 280 – 550 nm, after excitation in the 270 – 370 nm range. After normalization of the spectra, chemometric evaluation of the spectral data was carried out using principal component analysis (PCA). This study indicates that front-face fluorescence spectroscopy is a promising technique for the authentication of geographical origin of honey and may also be useful for determination of the botanical origin within the same unifloral honey type.