A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.
Keywords:
mangiferin / in vitro culture / gentiopicrine / Gentiana asclepiadea
Source:
Phyton - Annales Rei Botanicae, 2006, 46, 1, 45-54
TY - JOUR
AU - Dević, M.
AU - Momcilović, Ivana
AU - Krstić, D.
AU - Maksimović, Vuk
AU - Konjević, R.
PY - 2006
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/160
AB - A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.
T2 - Phyton - Annales Rei Botanicae
T1 - In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin
EP - 54
IS - 1
SP - 45
VL - 46
UR - https://hdl.handle.net/21.15107/rcub_rimsi_160
ER -
@article{
author = "Dević, M. and Momcilović, Ivana and Krstić, D. and Maksimović, Vuk and Konjević, R.",
year = "2006",
abstract = "A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.",
journal = "Phyton - Annales Rei Botanicae",
title = "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin",
pages = "54-45",
number = "1",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_160"
}
Dević, M., Momcilović, I., Krstić, D., Maksimović, V.,& Konjević, R.. (2006). In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton - Annales Rei Botanicae, 46(1), 45-54.
https://hdl.handle.net/21.15107/rcub_rimsi_160
Dević M, Momcilović I, Krstić D, Maksimović V, Konjević R. In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton - Annales Rei Botanicae. 2006;46(1):45-54.
https://hdl.handle.net/21.15107/rcub_rimsi_160 .
Dević, M., Momcilović, Ivana, Krstić, D., Maksimović, Vuk, Konjević, R., "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin" in Phyton - Annales Rei Botanicae, 46, no. 1 (2006):45-54,
https://hdl.handle.net/21.15107/rcub_rimsi_160 .
