Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples
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2022
Authors
Stanković, Mira
Bartolić, Dragana

Mutavdžić, Dragosav

Marković, Smilja

Grubic, Sasa
Jovanović, Nemanja M.

Radotić, Ksenija

Article (Published version)

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In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R (2) was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-...ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.
Keywords:
MCR-ALS method / infection / honey / fluorescence / differential scanning calorimetry / Apis melliferaSource:
Journal of Apicultural Research, 2022Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-200175)
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Stanković, Mira AU - Bartolić, Dragana AU - Mutavdžić, Dragosav AU - Marković, Smilja AU - Grubic, Sasa AU - Jovanović, Nemanja M. AU - Radotić, Ksenija PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1550 AB - In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R (2) was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Apicultural Research T1 - Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples DO - 10.1080/00218839.2021.1889803 ER -
@article{ author = "Stanković, Mira and Bartolić, Dragana and Mutavdžić, Dragosav and Marković, Smilja and Grubic, Sasa and Jovanović, Nemanja M. and Radotić, Ksenija", year = "2022", abstract = "In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R (2) was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Apicultural Research", title = "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples", doi = "10.1080/00218839.2021.1889803" }
Stanković, M., Bartolić, D., Mutavdžić, D., Marković, S., Grubic, S., Jovanović, N. M.,& Radotić, K.. (2022). Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research Taylor & Francis Ltd, Abingdon.. https://doi.org/10.1080/00218839.2021.1889803
Stanković M, Bartolić D, Mutavdžić D, Marković S, Grubic S, Jovanović NM, Radotić K. Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research. 2022;. doi:10.1080/00218839.2021.1889803 .
Stanković, Mira, Bartolić, Dragana, Mutavdžić, Dragosav, Marković, Smilja, Grubic, Sasa, Jovanović, Nemanja M., Radotić, Ksenija, "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples" in Journal of Apicultural Research (2022), https://doi.org/10.1080/00218839.2021.1889803 . .