A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).
Keywords:
TNP / scFv-antibody / new immunoreagent tool / fusion protein (scFv-antibody  / enzyme / enzyme of choice)
Source:
Natural Product Research, 2022, 36, 5, 1317-1320
Publisher:
Taylor & Francis Ltd, Abingdon
Funding / projects:
University Hamburg, Germany
German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD)
TY - JOUR
AU - Simonović, Mladen
AU - Mix, Thorsten
AU - Glumac, Miodrag
AU - Pejin, Boris
PY - 2022
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1547
AB - A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).
PB - Taylor & Francis Ltd, Abingdon
T2 - Natural Product Research
T1 - 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive
EP - 1320
IS - 5
SP - 1317
VL - 36
DO - 10.1080/14786419.2020.1860977
ER -
@article{
author = "Simonović, Mladen and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive",
pages = "1320-1317",
number = "5",
volume = "36",
doi = "10.1080/14786419.2020.1860977"
}
Simonović, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 36(5), 1317-1320.
https://doi.org/10.1080/14786419.2020.1860977
Simonović M, Mix T, Glumac M, Pejin B. 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. in Natural Product Research. 2022;36(5):1317-1320.
doi:10.1080/14786419.2020.1860977 .
Simonović, Mladen, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive" in Natural Product Research, 36, no. 5 (2022):1317-1320,
https://doi.org/10.1080/14786419.2020.1860977 . .
