Approach for patch-clamping using an upright microscope with z-axis movable stage
Samo za registrovane korisnike
2022
Autori
Peric, MinaBataveljic, Danijela
Bijelic, Dunja
Milicević, Katarina
Andjus, Pavle R.
Bogdanović Pristov, Jelena
Nikolić, Ljiljana M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be eas...ily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.
Ključne reči:
patch-clamp / microscopy / life science / glial cells / cellular physiologyIzvor:
Microscopy Research and Technique, 2022Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-200178)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-200053)
DOI: 10.1002/jemt.24066
ISSN: 1059-910X
PubMed: 35088507
WoS: 000747718200001
Scopus: 2-s2.0-85123799490
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Peric, Mina AU - Bataveljic, Danijela AU - Bijelic, Dunja AU - Milicević, Katarina AU - Andjus, Pavle R. AU - Bogdanović Pristov, Jelena AU - Nikolić, Ljiljana M. PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1546 AB - We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping. PB - Wiley, Hoboken T2 - Microscopy Research and Technique T1 - Approach for patch-clamping using an upright microscope with z-axis movable stage DO - 10.1002/jemt.24066 ER -
@article{ author = "Peric, Mina and Bataveljic, Danijela and Bijelic, Dunja and Milicević, Katarina and Andjus, Pavle R. and Bogdanović Pristov, Jelena and Nikolić, Ljiljana M.", year = "2022", abstract = "We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.", publisher = "Wiley, Hoboken", journal = "Microscopy Research and Technique", title = "Approach for patch-clamping using an upright microscope with z-axis movable stage", doi = "10.1002/jemt.24066" }
Peric, M., Bataveljic, D., Bijelic, D., Milicević, K., Andjus, P. R., Bogdanović Pristov, J.,& Nikolić, L. M.. (2022). Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique Wiley, Hoboken.. https://doi.org/10.1002/jemt.24066
Peric M, Bataveljic D, Bijelic D, Milicević K, Andjus PR, Bogdanović Pristov J, Nikolić LM. Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique. 2022;. doi:10.1002/jemt.24066 .
Peric, Mina, Bataveljic, Danijela, Bijelic, Dunja, Milicević, Katarina, Andjus, Pavle R., Bogdanović Pristov, Jelena, Nikolić, Ljiljana M., "Approach for patch-clamping using an upright microscope with z-axis movable stage" in Microscopy Research and Technique (2022), https://doi.org/10.1002/jemt.24066 . .