A novel and effective natural product-based immunodetection tool for TNT-like compounds
Nema prikaza
Autori
Simonović, MladenOstojic, Sanja
Micic, Darko
Bisercic-Savić, Marjetka
Mix, Thorsten
Glumac, Miodrag
Pejin, Boris
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies... and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .
Ključne reči:
TNP-Tris / beta-lactamase / 11B3-scFv-beta-lactamase / 11B3-scFv-antibodyIzvor:
Natural Product Research, 2022, 36, 3, 857-861Izdavač:
- Taylor & Francis Ltd, Abingdon
Finansiranje / projekti:
- German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD)
- Uticaj sorte i uslova gajenja na sadržaj bioaktivnih komponenti jagodastog i koštičavog voća i dobijanje biološki vrednih proizvoda poboljšanim i novim tehnologijama (RS-MESTD-Technological Development (TD or TR)-31093)
- Osmotska dehidratacija hrane - energetski i ekološki aspekti održive proizvodnje (RS-MESTD-Technological Development (TD or TR)-31055)
- Bioaktivni prirodni proizvodi samoniklih, gajenih i jestivih biljaka: određivanje struktura i aktivnosti (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1080/14786419.2020.1806269
ISSN: 1478-6419
PubMed: 32787566
WoS: 000558970500001
Scopus: 2-s2.0-85089443691
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Simonović, Mladen AU - Ostojic, Sanja AU - Micic, Darko AU - Bisercic-Savić, Marjetka AU - Mix, Thorsten AU - Glumac, Miodrag AU - Pejin, Boris PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1537 AB - This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] . PB - Taylor & Francis Ltd, Abingdon T2 - Natural Product Research T1 - A novel and effective natural product-based immunodetection tool for TNT-like compounds EP - 861 IS - 3 SP - 857 VL - 36 DO - 10.1080/14786419.2020.1806269 ER -
@article{ author = "Simonović, Mladen and Ostojic, Sanja and Micic, Darko and Bisercic-Savić, Marjetka and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris", year = "2022", abstract = "This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Natural Product Research", title = "A novel and effective natural product-based immunodetection tool for TNT-like compounds", pages = "861-857", number = "3", volume = "36", doi = "10.1080/14786419.2020.1806269" }
Simonović, M., Ostojic, S., Micic, D., Bisercic-Savić, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research Taylor & Francis Ltd, Abingdon., 36(3), 857-861. https://doi.org/10.1080/14786419.2020.1806269
Simonović M, Ostojic S, Micic D, Bisercic-Savić M, Mix T, Glumac M, Pejin B. A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research. 2022;36(3):857-861. doi:10.1080/14786419.2020.1806269 .
Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "A novel and effective natural product-based immunodetection tool for TNT-like compounds" in Natural Product Research, 36, no. 3 (2022):857-861, https://doi.org/10.1080/14786419.2020.1806269 . .