A novel and effective natural product-based immunodetection tool for TNT-like compounds
Нема приказа
Аутори
Simonović, MladenOstojic, Sanja
Micic, Darko
Bisercic-Savić, Marjetka
Mix, Thorsten
Glumac, Miodrag
Pejin, Boris
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies... and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .
Кључне речи:
TNP-Tris / beta-lactamase / 11B3-scFv-beta-lactamase / 11B3-scFv-antibodyИзвор:
Natural Product Research, 2022, 36, 3, 857-861Издавач:
- Taylor & Francis Ltd, Abingdon
Финансирање / пројекти:
- German Academic Exchange Service (DAAD)Deutscher Akademischer Austausch Dienst (DAAD)
- Утицај сорте и услова гајења на садржај биоактивних компоненти јагодастог и коштичавог воћа и добијање биолошки вредних производа побољшаним и новим технологијама (RS-MESTD-Technological Development (TD or TR)-31093)
- Осмотска дехидратација хране - енергетски и еколошки аспекти одрживе производње (RS-MESTD-Technological Development (TD or TR)-31055)
- Биоактивни природни производи самониклих, гајених и јестивих биљака: одређивање структура и активности (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1080/14786419.2020.1806269
ISSN: 1478-6419
PubMed: 32787566
WoS: 000558970500001
Scopus: 2-s2.0-85089443691
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Simonović, Mladen AU - Ostojic, Sanja AU - Micic, Darko AU - Bisercic-Savić, Marjetka AU - Mix, Thorsten AU - Glumac, Miodrag AU - Pejin, Boris PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1537 AB - This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] . PB - Taylor & Francis Ltd, Abingdon T2 - Natural Product Research T1 - A novel and effective natural product-based immunodetection tool for TNT-like compounds EP - 861 IS - 3 SP - 857 VL - 36 DO - 10.1080/14786419.2020.1806269 ER -
@article{ author = "Simonović, Mladen and Ostojic, Sanja and Micic, Darko and Bisercic-Savić, Marjetka and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris", year = "2022", abstract = "This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Natural Product Research", title = "A novel and effective natural product-based immunodetection tool for TNT-like compounds", pages = "861-857", number = "3", volume = "36", doi = "10.1080/14786419.2020.1806269" }
Simonović, M., Ostojic, S., Micic, D., Bisercic-Savić, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research Taylor & Francis Ltd, Abingdon., 36(3), 857-861. https://doi.org/10.1080/14786419.2020.1806269
Simonović M, Ostojic S, Micic D, Bisercic-Savić M, Mix T, Glumac M, Pejin B. A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research. 2022;36(3):857-861. doi:10.1080/14786419.2020.1806269 .
Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "A novel and effective natural product-based immunodetection tool for TNT-like compounds" in Natural Product Research, 36, no. 3 (2022):857-861, https://doi.org/10.1080/14786419.2020.1806269 . .