Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, ...paratopes might be located in the CDRH3 or VH regions.
TY - JOUR
AU - Srdic-Rajic, T.
AU - Metlas, R.
PY - 2022
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1522
AB - Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.
PB - Elsevier B.V.
T2 - Immunology Letters
T1 - Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location
EP - 57
SP - 55
VL - 241
DO - 10.1016/j.imlet.2021.11.003
ER -
@article{
author = "Srdic-Rajic, T. and Metlas, R.",
year = "2022",
abstract = "Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.",
publisher = "Elsevier B.V.",
journal = "Immunology Letters",
title = "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location",
pages = "57-55",
volume = "241",
doi = "10.1016/j.imlet.2021.11.003"
}
Srdic-Rajic, T.,& Metlas, R.. (2022). Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters
Elsevier B.V.., 241, 55-57.
https://doi.org/10.1016/j.imlet.2021.11.003
Srdic-Rajic T, Metlas R. Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters. 2022;241:55-57.
doi:10.1016/j.imlet.2021.11.003 .
Srdic-Rajic, T., Metlas, R., "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location" in Immunology Letters, 241 (2022):55-57,
https://doi.org/10.1016/j.imlet.2021.11.003 . .
