The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .
TY - JOUR
AU - Pejin, Boris
AU - Simonović, Mladen
AU - Talevska, Aleksandra
AU - Glumac, Miodrag
AU - Jakimov, Dimitar
AU - Kojić, Vesna
PY - 2021
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1511
AB - The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .
PB - Taylor & Francis Ltd, Abingdon
T2 - Natural Product Research
T1 - A neglected natural source for targeting glioblastoma
EP - 1860
IS - 11
SP - 1856
VL - 35
DO - 10.1080/14786419.2019.1638386
ER -
@article{
author = "Pejin, Boris and Simonović, Mladen and Talevska, Aleksandra and Glumac, Miodrag and Jakimov, Dimitar and Kojić, Vesna",
year = "2021",
abstract = "The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A neglected natural source for targeting glioblastoma",
pages = "1860-1856",
number = "11",
volume = "35",
doi = "10.1080/14786419.2019.1638386"
}
Pejin, B., Simonović, M., Talevska, A., Glumac, M., Jakimov, D.,& Kojić, V.. (2021). A neglected natural source for targeting glioblastoma. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 35(11), 1856-1860.
https://doi.org/10.1080/14786419.2019.1638386
Pejin B, Simonović M, Talevska A, Glumac M, Jakimov D, Kojić V. A neglected natural source for targeting glioblastoma. in Natural Product Research. 2021;35(11):1856-1860.
doi:10.1080/14786419.2019.1638386 .
Pejin, Boris, Simonović, Mladen, Talevska, Aleksandra, Glumac, Miodrag, Jakimov, Dimitar, Kojić, Vesna, "A neglected natural source for targeting glioblastoma" in Natural Product Research, 35, no. 11 (2021):1856-1860,
https://doi.org/10.1080/14786419.2019.1638386 . .
