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Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems
dc.creator | Prodanović, Radivoje | |
dc.creator | Milosavić, Nenad | |
dc.creator | Jovanović, Slobodan | |
dc.creator | Prodanović, Olivera | |
dc.creator | Ćirković-Velicković, Tanja | |
dc.creator | Vujcic, Zoran | |
dc.creator | Jankov, Ratko M. | |
dc.date.accessioned | 2022-04-05T14:08:08Z | |
dc.date.available | 2022-04-05T14:08:08Z | |
dc.date.issued | 2006 | |
dc.identifier.issn | 1024-2422 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/150 | |
dc.description.abstract | The activity of alpha-glucosidase from baker's yeast was determined in various concentrations of dioxan, tetrahydrofuran, tert-butanol, dimethylformamide, methanol and dimethylsulfoxide (DMSO). Higher activities were observed with sucrose than with nitrophenylglucoside as substrate in cosolvent mixtures. In 30% (v/v) DMSO, 25% of the activity obtained in pure water was detected, and in 30% (v/v) methanol 12.5% of the activity in pure water was detected, while in other cosolvents there was almost no activity under these conditions. alpha-glucosidase was immobilized onto a macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), by the glutaraldehyde method. By immobilization, the half-life of the enzyme in 35% (v/v) methanol was increased from 6 to 60min and from 4 to 15min in 45% (v/v) DMSO. The activity of the immobilized enzyme in 30% (v/v) DMSO and 30% (v/v) methanol was 22% and 18% of the activity in pure water, respectively. | en |
dc.publisher | Taylor & Francis Ltd, Abingdon | |
dc.rights | restrictedAccess | |
dc.source | Biocatalysis and Biotransformation | |
dc.subject | transglucosylation | en |
dc.subject | maltase | en |
dc.subject | macroporous | en |
dc.subject | glycidylmethacrylate | en |
dc.subject | glutaraldehyde | en |
dc.title | Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 200 | |
dc.citation.issue | 3 | |
dc.citation.other | 24(3): 195-200 | |
dc.citation.rank | M22 | |
dc.citation.spage | 195 | |
dc.citation.volume | 24 | |
dc.identifier.doi | 10.1080/10242420600655903 | |
dc.identifier.scopus | 2-s2.0-33748340626 | |
dc.identifier.wos | 000239382800003 | |
dc.type.version | publishedVersion |
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