Приказ основних података о документу

dc.creatorPopović, Nikolina
dc.creatorPrzulj, Dunja
dc.creatorMladenović, Maja
dc.creatorProdanović, Olivera
dc.creatorEce, Selin
dc.creatorIlic-Durdic, Karla
dc.creatorOstafe, Raluca
dc.creatorFischer, Rainer
dc.creatorProdanović, Radivoje
dc.date.accessioned2022-04-05T15:37:55Z
dc.date.available2022-04-05T15:37:55Z
dc.date.issued2021
dc.identifier.issn0141-8130
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/1490
dc.description.abstractHigh amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove dyes from the environment and wastewaters, researchers focused on applying immobilized enzymes due to mild reaction conditions and enzyme nontoxicity. Laccases are oxidases with wide substrate specificity, capable of degradation of many different dye types. Laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The specific activity of surface-displayed laccase was increased by toluene-induced lysis to 3.1 U/g of cell walls. For cell wall laccase immobilization within hydrogel beads, alginate was modified by dopamine using periodate oxidation and reductive amination and characterized by UV-Vis, FTIR, and NMR spectroscopy. Cell wall laccase was immobilized within alginate and dopamine-alginate beads additionally cross-linked by oxygen and laccase. The immobilized enzyme's specific activity was two times higher using dopamine-alginate compared to native alginate beads, and immobilization yield increased 16 times. Cell wall laccase immobilized within dopamine-alginate beads decolorized Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue with 100% efficiency and after ten rounds of multiple-use retained decolorization efficiency of 90% with Evans Blue and 61% with Amido Black.en
dc.publisherElsevier, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200053/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Biological Macromolecules
dc.subjectYeast surface displayen
dc.subjectLaccaseen
dc.subjectDopamineen
dc.titleImmobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorizationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1080
dc.citation.other181: 1072-1080
dc.citation.rankaM21~
dc.citation.spage1072
dc.citation.volume181
dc.identifier.doi10.1016/j.ijbiomac.2021.04.115
dc.identifier.pmid33892032
dc.identifier.scopus2-s2.0-85104701498
dc.identifier.wos000656916800003
dc.type.versionpublishedVersion


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Приказ основних података о документу