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dc.creatorMarković, Sanja
dc.creatorStanković, Slaviša
dc.creatorIlicic, Renata
dc.creatorVeljović-Jovanović, Sonja
dc.creatorMilić Komić, Sonja
dc.creatorJelušić, Aleksandra
dc.creatorPopović, Tatjana
dc.date.accessioned2022-04-05T15:34:22Z
dc.date.available2022-04-05T15:34:22Z
dc.date.issued2021
dc.identifier.issn0032-0862
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/1438
dc.description.abstractSince 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200053/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200010/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200178/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200117/RS//
dc.rightsrestrictedAccess
dc.sourcePlant Pathology
dc.subjectTCS networken
dc.subjectPOD isoformsen
dc.subjectPOD activityen
dc.subjectMLSAen
dc.subjectbrown roten
dc.titleRalstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubersen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1959
dc.citation.issue8
dc.citation.other70(8): 1945-1959
dc.citation.rankM21
dc.citation.spage1945
dc.citation.volume70
dc.identifier.doi10.1111/ppa.13421
dc.identifier.scopus2-s2.0-85109136733
dc.identifier.wos000670096700001
dc.type.versionpublishedVersion


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