Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts

2021
Аутори
Simonović Radosavljević, Jasna
Mitrović, Aleksandra

Radotić, Ksenija

Zimanyi, Laszlo

Garab, Gyozo

Steinbach, Gabor

Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarizat...ion spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.
Кључне речи:
RCM / polarized light / molecular organization / micro-spectropolarimetry / fluorescence-detected linear dichroism / DP-LSM / circular dichroism / chloroplast / cell wall / anisotropyИзвор:
International Journal of Molecular Sciences, 2021, 22, 14Издавач:
- MDPI, Basel
Финансирање / пројекти:
- Hungarian Ministry for National Economy (Economic Development and Innovation Operational Programme) [GINOP-2.3.3-15-201600003, GINOP-2.3.3-15-2016-00030, GINOP-2.1.7-15-2016-00713]
- Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-200053)
- National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 128679]
- GINOP-2.3.2-15-2016-00001]
DOI: 10.3390/ijms22147661
ISSN: 1422-0067
PubMed: 34299279
WoS: 000676684200001
Scopus: 2-s2.0-85110246274
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Simonović Radosavljević, Jasna AU - Mitrović, Aleksandra AU - Radotić, Ksenija AU - Zimanyi, Laszlo AU - Garab, Gyozo AU - Steinbach, Gabor PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1427 AB - Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies. PB - MDPI, Basel T2 - International Journal of Molecular Sciences T1 - Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts IS - 14 VL - 22 DO - 10.3390/ijms22147661 ER -
@article{ author = "Simonović Radosavljević, Jasna and Mitrović, Aleksandra and Radotić, Ksenija and Zimanyi, Laszlo and Garab, Gyozo and Steinbach, Gabor", year = "2021", abstract = "Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.", publisher = "MDPI, Basel", journal = "International Journal of Molecular Sciences", title = "Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts", number = "14", volume = "22", doi = "10.3390/ijms22147661" }
Simonović Radosavljević, J., Mitrović, A., Radotić, K., Zimanyi, L., Garab, G.,& Steinbach, G.. (2021). Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts. in International Journal of Molecular Sciences MDPI, Basel., 22(14). https://doi.org/10.3390/ijms22147661
Simonović Radosavljević J, Mitrović A, Radotić K, Zimanyi L, Garab G, Steinbach G. Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts. in International Journal of Molecular Sciences. 2021;22(14). doi:10.3390/ijms22147661 .
Simonović Radosavljević, Jasna, Mitrović, Aleksandra, Radotić, Ksenija, Zimanyi, Laszlo, Garab, Gyozo, Steinbach, Gabor, "Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts" in International Journal of Molecular Sciences, 22, no. 14 (2021), https://doi.org/10.3390/ijms22147661 . .