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Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates

Authorized Users Only
2021
Authors
Jelušić, Aleksandra
Popović, Tatjana
Dimkić, Ivica
Mitrović, Petar
Peeters, Kelly
Miklavcic-Visnjevec, Ana
Tavzes, Crtomir
Stanković, Slaviša
Berić, Tanja
Article (Published version)
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Abstract
Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests o...n winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.

Keywords:
Volatile organic compounds / Pseudomonas orientalis / Metabarcoding / Lipopeptides / Bacillus velezensis / Bacillus megaterium
Source:
Biological Control, 2021, 160
Publisher:
  • Academic Press Inc Elsevier Science, San Diego
Funding / projects:
  • European CommissionEuropean CommissionEuropean Commission Joint Research Centre [739574]
  • ARRSSlovenian Research Agency - Slovenia [J4-1767]
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-200010)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200178 (University of Belgrade, Faculty of Biology) (RS-200178)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-200032)

DOI: 10.1016/j.biocontrol.2021.104695

ISSN: 1049-9644

WoS: 000681066200013

Scopus: 2-s2.0-85109621719
[ Google Scholar ]
4
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1426
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavcic-Visnjevec, Ana
AU  - Tavzes, Crtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1426
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 
@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavcic-Visnjevec, Ana and Tavzes, Crtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}
Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavcic-Visnjevec, A., Tavzes, C., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 160.
https://doi.org/10.1016/j.biocontrol.2021.104695
Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavcic-Visnjevec A, Tavzes C, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160.
doi:10.1016/j.biocontrol.2021.104695 .
Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavcic-Visnjevec, Ana, Tavzes, Crtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021),
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

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