Spatial distribution of apoplastic antioxidative constituents in maize root
Само за регистроване кориснике
2021
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showe...d a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue.
Кључне речи:
apoplast / ascorbate oxidase / electron paramagnetic resonance spectroscopy / organic acids / peroxidase / phenolics / sugars / superoxide dismutaseИзвор:
Physiologia Plantarum, 2021, 173, 3, 818-828Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200146 (Универзитет у Београду, Факултет за физичку хемију) (RS-MESTD-inst-2020-200146)
DOI: 10.1111/ppl.13476
ISSN: 0031-9317
PubMed: 34109632
WoS: 000663679500001
Scopus: 2-s2.0-85108365280
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Dragišić Maksimović, Jelena AU - Mojović, Miloš AU - Vučinić, Željko AU - Maksimović, Vuk PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1424 AB - Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue. PB - Wiley, Hoboken T2 - Physiologia Plantarum T1 - Spatial distribution of apoplastic antioxidative constituents in maize root EP - 828 IS - 3 SP - 818 VL - 173 DO - 10.1111/ppl.13476 ER -
@article{ author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Vučinić, Željko and Maksimović, Vuk", year = "2021", abstract = "Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue.", publisher = "Wiley, Hoboken", journal = "Physiologia Plantarum", title = "Spatial distribution of apoplastic antioxidative constituents in maize root", pages = "828-818", number = "3", volume = "173", doi = "10.1111/ppl.13476" }
Dragišić Maksimović, J., Mojović, M., Vučinić, Ž.,& Maksimović, V.. (2021). Spatial distribution of apoplastic antioxidative constituents in maize root. in Physiologia Plantarum Wiley, Hoboken., 173(3), 818-828. https://doi.org/10.1111/ppl.13476
Dragišić Maksimović J, Mojović M, Vučinić Ž, Maksimović V. Spatial distribution of apoplastic antioxidative constituents in maize root. in Physiologia Plantarum. 2021;173(3):818-828. doi:10.1111/ppl.13476 .
Dragišić Maksimović, Jelena, Mojović, Miloš, Vučinić, Željko, Maksimović, Vuk, "Spatial distribution of apoplastic antioxidative constituents in maize root" in Physiologia Plantarum, 173, no. 3 (2021):818-828, https://doi.org/10.1111/ppl.13476 . .