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An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes

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2005
139.pdf (131.9Kb)
Authors
Mojović, Miloš
Spasojević, Ivan
Vuletic, M
Vučinić, Željko
Bačić, Goran
Article (Published version)
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Abstract
Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in... free radical studies of biological systems, with a number of advantages inherent to them.

Keywords:
superoxide / spin-trap / spin-probe / maize plasma membrane / hydroxyl radical / EPR
Source:
Journal of the Serbian Chemical Society, 2005, 70, 2, 177-186
Publisher:
  • Srpsko hemijsko društvo, Beograd

DOI: 10.2298/JSC0502177M

ISSN: 0352-5139

WoS: 000228072300005

Scopus: 2-s2.0-25144441582
[ Google Scholar ]
10
12
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/142
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Vuletic, M
AU  - Vučinić, Željko
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/142
AB  - Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes
EP  - 186
IS  - 2
SP  - 177
VL  - 70
DO  - 10.2298/JSC0502177M
ER  - 
@article{
author = "Mojović, Miloš and Spasojević, Ivan and Vuletic, M and Vučinić, Željko and Bačić, Goran",
year = "2005",
abstract = "Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes",
pages = "186-177",
number = "2",
volume = "70",
doi = "10.2298/JSC0502177M"
}
Mojović, M., Spasojević, I., Vuletic, M., Vučinić, Ž.,& Bačić, G.. (2005). An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(2), 177-186.
https://doi.org/10.2298/JSC0502177M
Mojović M, Spasojević I, Vuletic M, Vučinić Ž, Bačić G. An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society. 2005;70(2):177-186.
doi:10.2298/JSC0502177M .
Mojović, Miloš, Spasojević, Ivan, Vuletic, M, Vučinić, Željko, Bačić, Goran, "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes" in Journal of the Serbian Chemical Society, 70, no. 2 (2005):177-186,
https://doi.org/10.2298/JSC0502177M . .

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