Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence
Само за регистроване кориснике
2020
Чланак у часопису (Објављена верзија)

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The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in... both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.
Кључне речи:
X-ray fluorescence microscopy / Populus alba L. / Phosphorus homeostasis / Phosphates / P-31 NMR spectroscopy / Intracellular phosphate distributionИзвор:
Environmental Science and Pollution Research, 2020, 27, 3, 3320-3328Издавач:
- Springer Heidelberg, Heidelberg
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-173017)
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-173040)
DOI: 10.1007/s11356-019-07200-9
ISSN: 0944-1344
PubMed: 31838696
WoS: 000514849100069
Scopus: 2-s2.0-85076863203
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Zakrzewska, Joanna AU - Mitrović, Aleksandra AU - Mutavdžić, Dragosav AU - Ducic, Tanja AU - Radotić, Ksenija PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1394 AB - The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation. PB - Springer Heidelberg, Heidelberg T2 - Environmental Science and Pollution Research T1 - Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence EP - 3328 IS - 3 SP - 3320 VL - 27 DO - 10.1007/s11356-019-07200-9 ER -
@article{ author = "Zakrzewska, Joanna and Mitrović, Aleksandra and Mutavdžić, Dragosav and Ducic, Tanja and Radotić, Ksenija", year = "2020", abstract = "The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.", publisher = "Springer Heidelberg, Heidelberg", journal = "Environmental Science and Pollution Research", title = "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence", pages = "3328-3320", number = "3", volume = "27", doi = "10.1007/s11356-019-07200-9" }
Zakrzewska, J., Mitrović, A., Mutavdžić, D., Ducic, T.,& Radotić, K.. (2020). Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research Springer Heidelberg, Heidelberg., 27(3), 3320-3328. https://doi.org/10.1007/s11356-019-07200-9
Zakrzewska J, Mitrović A, Mutavdžić D, Ducic T, Radotić K. Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research. 2020;27(3):3320-3328. doi:10.1007/s11356-019-07200-9 .
Zakrzewska, Joanna, Mitrović, Aleksandra, Mutavdžić, Dragosav, Ducic, Tanja, Radotić, Ksenija, "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence" in Environmental Science and Pollution Research, 27, no. 3 (2020):3320-3328, https://doi.org/10.1007/s11356-019-07200-9 . .