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Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence

Authorized Users Only
2020
Authors
Zakrzewska, Joanna
Mitrović, Aleksandra
Mutavdžić, Dragosav
Ducic, Tanja
Radotić, Ksenija
Article (Published version)
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Abstract
The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in... both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.

Keywords:
X-ray fluorescence microscopy / Populus alba L. / Phosphorus homeostasis / Phosphates / P-31 NMR spectroscopy / Intracellular phosphate distribution
Source:
Environmental Science and Pollution Research, 2020, 27, 3, 3320-3328
Publisher:
  • Springer Heidelberg, Heidelberg
Funding / projects:
  • Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
  • The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-173040)

DOI: 10.1007/s11356-019-07200-9

ISSN: 0944-1344

PubMed: 31838696

WoS: 000514849100069

Scopus: 2-s2.0-85076863203
[ Google Scholar ]
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1394
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Zakrzewska, Joanna
AU  - Mitrović, Aleksandra
AU  - Mutavdžić, Dragosav
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1394
AB  - The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence
EP  - 3328
IS  - 3
SP  - 3320
VL  - 27
DO  - 10.1007/s11356-019-07200-9
ER  - 
@article{
author = "Zakrzewska, Joanna and Mitrović, Aleksandra and Mutavdžić, Dragosav and Ducic, Tanja and Radotić, Ksenija",
year = "2020",
abstract = "The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence",
pages = "3328-3320",
number = "3",
volume = "27",
doi = "10.1007/s11356-019-07200-9"
}
Zakrzewska, J., Mitrović, A., Mutavdžić, D., Ducic, T.,& Radotić, K.. (2020). Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(3), 3320-3328.
https://doi.org/10.1007/s11356-019-07200-9
Zakrzewska J, Mitrović A, Mutavdžić D, Ducic T, Radotić K. Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research. 2020;27(3):3320-3328.
doi:10.1007/s11356-019-07200-9 .
Zakrzewska, Joanna, Mitrović, Aleksandra, Mutavdžić, Dragosav, Ducic, Tanja, Radotić, Ksenija, "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence" in Environmental Science and Pollution Research, 27, no. 3 (2020):3320-3328,
https://doi.org/10.1007/s11356-019-07200-9 . .

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