The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.
Keywords:
polyphosphates / Phycomyces / P-31 NMR / anoxia
Source:
Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 2005, 1048, 482-486
TY - JOUR
AU - Zakrzewska, Joanna
AU - Žižić, Milan
AU - Zivić, Miroslav
PY - 2005
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/139
AB - The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.
PB - New York Acad Sciences, New York
T2 - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1 - The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy
EP - 486
SP - 482
VL - 1048
DO - 10.1196/annals.1342.073
ER -
@article{
author = "Zakrzewska, Joanna and Žižić, Milan and Zivić, Miroslav",
year = "2005",
abstract = "The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy",
pages = "486-482",
volume = "1048",
doi = "10.1196/annals.1342.073"
}
Zakrzewska, J., Žižić, M.,& Zivić, M.. (2005). The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 482-486.
https://doi.org/10.1196/annals.1342.073
Zakrzewska J, Žižić M, Zivić M. The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:482-486.
doi:10.1196/annals.1342.073 .
Zakrzewska, Joanna, Žižić, Milan, Zivić, Miroslav, "The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):482-486,
https://doi.org/10.1196/annals.1342.073 . .
