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dc.creatorMalembic-Maher, Sylvie
dc.creatorDesque, Delphine
dc.creatorKhalil, Dima
dc.creatorSalar, Pascal
dc.creatorBergey, Bernard
dc.creatorDanet, Jean-Luc
dc.creatorDuret, Sybille
dc.creatorDubrana-Ourabah, Marie-Pierre
dc.creatorBeven, Laure
dc.creatorEmber, Ibolya
dc.creatorAcs, Zoltan
dc.creatorDella, Bartola, Michele
dc.creatorMaterazzi, Alberto
dc.creatorFilippin, Luisa
dc.creatorKrnjajić, Slobodan
dc.creatorKrstic, Oliver
dc.creatorTosevski, Ivo
dc.creatorLang, Friederike
dc.creatorJarausch, Barbara
dc.creatorKolber, Maria
dc.creatorJović, Jelena
dc.creatorAngelini, Elisa
dc.creatorArricau-Bouvery, Nathalie
dc.creatorMaixner, Michael
dc.creatorFoissac, Xavier
dc.date.accessioned2022-04-05T15:31:03Z
dc.date.available2022-04-05T15:31:03Z
dc.date.issued2020
dc.identifier.issn1553-7366
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/1389
dc.description.abstractAuthor summary Since the first outbreaks, Flavescence doree epidemics had been associated to the introduction of the North American leafhopper vector Scaphoideus titanus. We hereby show that the associated phytoplasma originated from European alders and that epidemics in grapevine are restricted to some phytoplasma genetic variants pre-existing in this wild asymptomatic plant host. The compatibility of this phytoplasma to the introduced S. titanus insect vector resulted from the adaptation of phytoplasma variable membrane proteins Vmps to leafhoppers of the same subfamily living on alders. Vmps organization is similar to adhesion related proteins (ARP) and seems to allow the duplication of pre-adapted repeated domains. This suggests a key role of the Vmp adhesins in the life-style of phytoplasmas infecting woody hosts that rely on the adaptation to new insect vectors to expand their plant-host range. Flavescence doree (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose, a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in clematis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and -III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and-III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster II VmpA promoted enhanced adhesion to the Deltocephalinae Euscelidius variegatus epithelial cells and were better retained in both E. variegatus and S. titanus midguts. Our data demonstrate that most FD phytoplasmas are endemic to European alders. Their emergence as grapevine epidemic pathogens appeared restricted to some genetic variants pre-existing in alders, whose compatibility to S. titanus correlates with different vmp gene sequences and VmpA binding properties.en
dc.publisherPublic Library Science, San Francisco
dc.relationEuropean South Eastern European SEE-ERANET Balkan Initiative network "Global epidemiology of phytoplasma diseases of economic importance in Southeast Europe" grant [06-100003110724]
dc.relationInterprofessional Council of Bordeaux' Wine (CIVB) grants [28299, 41044, 6099]
dc.relationINRAE Department of Plant Health and Environment (SPE) grant
dc.relationInterprofessional Bureau of Burgundy Wines (BIVB) grant [FD-ORIGIN1, FD-ORIGIN2]
dc.relationINRA Metaprogramme Sustainable Management of Crop Health (SMACH) grant
dc.relationBilateral French - Hungarian intergovernmental ST program [TET_11_FR-VINEPHYT-HU]
dc.relationbilateral French - Hungarian intergovernmental PHC BALATON grant [27853]
dc.relationBilateral French - Serbian intergovernmental PAVLE-SLAVIC grant [23624PG]
dc.relationGerman Academic Exchange Service in the frame of the French-German bilateral exchange program, PROCOPE [57049025]
dc.relationEuropean COST action [FA0807, COST-STSM-FA0807-7468, COST-STSMFA0807-05285]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43010/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePlos Pathogens
dc.subjectFlavescence doree / Scaphoideus titanus / Clematis vitalba / insect vector / quarantine disease / genetic diversity / vmp gene sequences
dc.titleWhen a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence doree epidemics in Europeen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue3
dc.citation.other16(3): -
dc.citation.rankaM21
dc.citation.volume16
dc.identifier.doi10.1371/journal.ppat.1007967
dc.identifier.fulltexthttp://rimsi.imsi.bg.ac.rs/bitstream/id/323/1386.pdf
dc.identifier.pmid32210479
dc.identifier.scopus2-s2.0-85082810210
dc.identifier.wos000523706200019
dc.type.versionpublishedVersion


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