Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
Само за регистроване кориснике
2020
Аутори
Cvetić-Antić, TijanaJanosević, Dusica
Maksimović, Vuk
Zivić, Miroslav
Budimir, Snežana M
Glamočlija, Jasmina
Mitrović, Aleksandra Lj.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization... and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
Кључне речи:
Transition from biotrophy to necrotrophy / Stomata / Plant antioxidative system / Fusaric acid / Fungal leaf infectionИзвор:
Journal of Plant Physiology, 2020, 244Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
DOI: 10.1016/j.jplph.2019.153086
ISSN: 0176-1617
PubMed: 31812905
WoS: 000504033800002
Scopus: 2-s2.0-85077225206
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Cvetić-Antić, Tijana AU - Janosević, Dusica AU - Maksimović, Vuk AU - Zivić, Miroslav AU - Budimir, Snežana M AU - Glamočlija, Jasmina AU - Mitrović, Aleksandra Lj. PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1383 AB - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides. PB - Elsevier Gmbh, Munich T2 - Journal of Plant Physiology T1 - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro VL - 244 DO - 10.1016/j.jplph.2019.153086 ER -
@article{ author = "Cvetić-Antić, Tijana and Janosević, Dusica and Maksimović, Vuk and Zivić, Miroslav and Budimir, Snežana M and Glamočlija, Jasmina and Mitrović, Aleksandra Lj.", year = "2020", abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.", publisher = "Elsevier Gmbh, Munich", journal = "Journal of Plant Physiology", title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro", volume = "244", doi = "10.1016/j.jplph.2019.153086" }
Cvetić-Antić, T., Janosević, D., Maksimović, V., Zivić, M., Budimir, S. M., Glamočlija, J.,& Mitrović, A. Lj.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology Elsevier Gmbh, Munich., 244. https://doi.org/10.1016/j.jplph.2019.153086
Cvetić-Antić T, Janosević D, Maksimović V, Zivić M, Budimir SM, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244. doi:10.1016/j.jplph.2019.153086 .
Cvetić-Antić, Tijana, Janosević, Dusica, Maksimović, Vuk, Zivić, Miroslav, Budimir, Snežana M, Glamočlija, Jasmina, Mitrović, Aleksandra Lj., "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020), https://doi.org/10.1016/j.jplph.2019.153086 . .