A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.
TY - JOUR
AU - Veljović-Jovanović, Sonja
AU - Kukavica, Biljana
AU - Cvetic, T
AU - Mojović, Miloš
AU - Vučinić, Željko
PY - 2005
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/138
AB - A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.
PB - New York Acad Sciences, New York
T2 - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1 - Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy
EP - 504
SP - 500
VL - 1048
DO - 10.1196/annals.1342.076
ER -
@article{
author = "Veljović-Jovanović, Sonja and Kukavica, Biljana and Cvetic, T and Mojović, Miloš and Vučinić, Željko",
year = "2005",
abstract = "A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy",
pages = "504-500",
volume = "1048",
doi = "10.1196/annals.1342.076"
}
Veljović-Jovanović, S., Kukavica, B., Cvetic, T., Mojović, M.,& Vučinić, Ž.. (2005). Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 500-504.
https://doi.org/10.1196/annals.1342.076
Veljović-Jovanović S, Kukavica B, Cvetic T, Mojović M, Vučinić Ž. Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:500-504.
doi:10.1196/annals.1342.076 .
Veljović-Jovanović, Sonja, Kukavica, Biljana, Cvetic, T, Mojović, Miloš, Vučinić, Željko, "Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):500-504,
https://doi.org/10.1196/annals.1342.076 . .
