Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship
Samo za registrovane korisnike
2020
Autori
Bartolić, DraganaMaksimović, Vuk
Dragišić Maksimović, Jelena
Stanković, Mira
Krstović, Sasa
Baosic, Rada
Radotić, Ksenija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the rol...es of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
Ključne reči:
total phenolic content (TPC) / total antioxidant capacity (TAA) / risk assessment / polyamine conjugates / maize (Zea mays L / dose-response / aflatoxin B1 (AFB1)Izvor:
Journal of the Science of Food and Agriculture, 2020, 100, 7, 2905-2910Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (RS-MESTD-Basic Research (BR or ON)-173040)
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-MESTD-Basic Research (BR or ON)-173017)
DOI: 10.1002/jsfa.10317
ISSN: 0022-5142
PubMed: 32031245
WoS: 000524970100011
Scopus: 2-s2.0-85082332251
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bartolić, Dragana AU - Maksimović, Vuk AU - Dragišić Maksimović, Jelena AU - Stanković, Mira AU - Krstović, Sasa AU - Baosic, Rada AU - Radotić, Ksenija PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1358 AB - BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality. PB - Wiley, Hoboken T2 - Journal of the Science of Food and Agriculture T1 - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship EP - 2910 IS - 7 SP - 2905 VL - 100 DO - 10.1002/jsfa.10317 ER -
@article{ author = "Bartolić, Dragana and Maksimović, Vuk and Dragišić Maksimović, Jelena and Stanković, Mira and Krstović, Sasa and Baosic, Rada and Radotić, Ksenija", year = "2020", abstract = "BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.", publisher = "Wiley, Hoboken", journal = "Journal of the Science of Food and Agriculture", title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship", pages = "2910-2905", number = "7", volume = "100", doi = "10.1002/jsfa.10317" }
Bartolić, D., Maksimović, V., Dragišić Maksimović, J., Stanković, M., Krstović, S., Baosic, R.,& Radotić, K.. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture Wiley, Hoboken., 100(7), 2905-2910. https://doi.org/10.1002/jsfa.10317
Bartolić D, Maksimović V, Dragišić Maksimović J, Stanković M, Krstović S, Baosic R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910. doi:10.1002/jsfa.10317 .
Bartolić, Dragana, Maksimović, Vuk, Dragišić Maksimović, Jelena, Stanković, Mira, Krstović, Sasa, Baosic, Rada, Radotić, Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship" in Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910, https://doi.org/10.1002/jsfa.10317 . .