DNA Sequencing with Single-Stranded DNA Rectification in a Nanogap Gated by N-Terminated Carbon Nanotube Electrodes
Само за регистроване кориснике
2020
Аутори
Djurišić, IvanaDražić, Miloš
Tomović, Aleksandar
Spasenović, Marko
Sljivancanin, Zeljko
Jovanović, Vladimir P.
Žikić, Radomir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fast, reliable, and inexpensive DNA sequencing is an important pursuit in healthcare, especially in personalized medicine with possible deep societal impacts. Despite significant progress in various nanopore-based sequencing configurations, challenges that remain in resolution and chromosome-size-long readout call for new approaches. Here we found strong rectification in the transversal current during single-stranded DNA translocation through a nanopore with side-embedded N-terminated carbon nanotube electrodes. Employing density functional theory and nonequilibrium Green's function formalisms, we show that the rectifying ratio (response to square pulses of alternating bias) bears high nucleobase specificity. Rectification arises because of bias-dependent resistance asymmetry on the deoxyribonucleotide-electrode interfaces. The asymmetry induces molecular charging and highest occupied molecular orbital pinning to the electrochemical potential of one of the electrodes, assisted by an in...-gap electric-field effect caused by dipoles at the terminated electrode ends. We propose the rectifying ratio, due to its order-of-magnitude-difference nucleobase selectivity and robustness to electrode-molecule orientation, as a promising readout quantifier for single-base resolution and chromosome-size-long single-read DNA sequencing. The proposed configurations are within experimental reach from the viewpoint of both nanofabrication and small current measurement.
Кључне речи:
nonequilibrium Green's function / nanogap / local gating / field effect / DNA sequencing / density functional theory / current rectificationИзвор:
Acs Applied Nano Materials, 2020, 3, 3, 3034-3043Издавач:
- Amer Chemical Soc, Washington
Финансирање / пројекти:
- Swiss National Science Foundation (SCOPES Project)Swiss National Science Foundation (SNSF) [152406]
- FP7-NMP, project acronym nanoDNAsequencing [GA214840]
- Електронске, транспортне и оптичке особине нанофазних материјала (RS-MESTD-Basic Research (BR or ON)-171033)
- Интегрална студија идентификације регионалних генетских фактора ризика и фактора ризика животне средине за масовне незаразне болести хумане популације у Србији - INGEMA_S (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41028)
DOI: 10.1021/acsanm.0c00385
ISSN: 2574-0970
WoS: 000526396200097
Scopus: 2-s2.0-85088388401
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Djurišić, Ivana AU - Dražić, Miloš AU - Tomović, Aleksandar AU - Spasenović, Marko AU - Sljivancanin, Zeljko AU - Jovanović, Vladimir P. AU - Žikić, Radomir PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1346 AB - Fast, reliable, and inexpensive DNA sequencing is an important pursuit in healthcare, especially in personalized medicine with possible deep societal impacts. Despite significant progress in various nanopore-based sequencing configurations, challenges that remain in resolution and chromosome-size-long readout call for new approaches. Here we found strong rectification in the transversal current during single-stranded DNA translocation through a nanopore with side-embedded N-terminated carbon nanotube electrodes. Employing density functional theory and nonequilibrium Green's function formalisms, we show that the rectifying ratio (response to square pulses of alternating bias) bears high nucleobase specificity. Rectification arises because of bias-dependent resistance asymmetry on the deoxyribonucleotide-electrode interfaces. The asymmetry induces molecular charging and highest occupied molecular orbital pinning to the electrochemical potential of one of the electrodes, assisted by an in-gap electric-field effect caused by dipoles at the terminated electrode ends. We propose the rectifying ratio, due to its order-of-magnitude-difference nucleobase selectivity and robustness to electrode-molecule orientation, as a promising readout quantifier for single-base resolution and chromosome-size-long single-read DNA sequencing. The proposed configurations are within experimental reach from the viewpoint of both nanofabrication and small current measurement. PB - Amer Chemical Soc, Washington T2 - Acs Applied Nano Materials T1 - DNA Sequencing with Single-Stranded DNA Rectification in a Nanogap Gated by N-Terminated Carbon Nanotube Electrodes EP - 3043 IS - 3 SP - 3034 VL - 3 DO - 10.1021/acsanm.0c00385 ER -
@article{ author = "Djurišić, Ivana and Dražić, Miloš and Tomović, Aleksandar and Spasenović, Marko and Sljivancanin, Zeljko and Jovanović, Vladimir P. and Žikić, Radomir", year = "2020", abstract = "Fast, reliable, and inexpensive DNA sequencing is an important pursuit in healthcare, especially in personalized medicine with possible deep societal impacts. Despite significant progress in various nanopore-based sequencing configurations, challenges that remain in resolution and chromosome-size-long readout call for new approaches. Here we found strong rectification in the transversal current during single-stranded DNA translocation through a nanopore with side-embedded N-terminated carbon nanotube electrodes. Employing density functional theory and nonequilibrium Green's function formalisms, we show that the rectifying ratio (response to square pulses of alternating bias) bears high nucleobase specificity. Rectification arises because of bias-dependent resistance asymmetry on the deoxyribonucleotide-electrode interfaces. The asymmetry induces molecular charging and highest occupied molecular orbital pinning to the electrochemical potential of one of the electrodes, assisted by an in-gap electric-field effect caused by dipoles at the terminated electrode ends. We propose the rectifying ratio, due to its order-of-magnitude-difference nucleobase selectivity and robustness to electrode-molecule orientation, as a promising readout quantifier for single-base resolution and chromosome-size-long single-read DNA sequencing. The proposed configurations are within experimental reach from the viewpoint of both nanofabrication and small current measurement.", publisher = "Amer Chemical Soc, Washington", journal = "Acs Applied Nano Materials", title = "DNA Sequencing with Single-Stranded DNA Rectification in a Nanogap Gated by N-Terminated Carbon Nanotube Electrodes", pages = "3043-3034", number = "3", volume = "3", doi = "10.1021/acsanm.0c00385" }
Djurišić, I., Dražić, M., Tomović, A., Spasenović, M., Sljivancanin, Z., Jovanović, V. P.,& Žikić, R.. (2020). DNA Sequencing with Single-Stranded DNA Rectification in a Nanogap Gated by N-Terminated Carbon Nanotube Electrodes. in Acs Applied Nano Materials Amer Chemical Soc, Washington., 3(3), 3034-3043. https://doi.org/10.1021/acsanm.0c00385
Djurišić I, Dražić M, Tomović A, Spasenović M, Sljivancanin Z, Jovanović VP, Žikić R. DNA Sequencing with Single-Stranded DNA Rectification in a Nanogap Gated by N-Terminated Carbon Nanotube Electrodes. in Acs Applied Nano Materials. 2020;3(3):3034-3043. doi:10.1021/acsanm.0c00385 .
Djurišić, Ivana, Dražić, Miloš, Tomović, Aleksandar, Spasenović, Marko, Sljivancanin, Zeljko, Jovanović, Vladimir P., Žikić, Radomir, "DNA Sequencing with Single-Stranded DNA Rectification in a Nanogap Gated by N-Terminated Carbon Nanotube Electrodes" in Acs Applied Nano Materials, 3, no. 3 (2020):3034-3043, https://doi.org/10.1021/acsanm.0c00385 . .