Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol
Abstract
The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.
Keywords:
Liposomes / EPR / Dysidea avara / avarol / antioxidantSource:
Journal of Liposome Research, 2020, 30, 3, 218-226Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Biomarkers in neurodegenerative and malignant processes (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1080/08982104.2019.1625378
ISSN: 0898-2104
PubMed: 31146614
WoS: 000472436900001
Scopus: 2-s2.0-85067698469
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Nakarada, Dura AU - Pejin, Boris AU - Tommonaro, Giuseppina AU - Mojović, Miloš PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1340 AB - The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Liposome Research T1 - Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol EP - 226 IS - 3 SP - 218 VL - 30 DO - 10.1080/08982104.2019.1625378 ER -
@article{ author = "Nakarada, Dura and Pejin, Boris and Tommonaro, Giuseppina and Mojović, Miloš", year = "2020", abstract = "The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Liposome Research", title = "Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol", pages = "226-218", number = "3", volume = "30", doi = "10.1080/08982104.2019.1625378" }
Nakarada, D., Pejin, B., Tommonaro, G.,& Mojović, M.. (2020). Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol. in Journal of Liposome Research Taylor & Francis Ltd, Abingdon., 30(3), 218-226. https://doi.org/10.1080/08982104.2019.1625378
Nakarada D, Pejin B, Tommonaro G, Mojović M. Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol. in Journal of Liposome Research. 2020;30(3):218-226. doi:10.1080/08982104.2019.1625378 .
Nakarada, Dura, Pejin, Boris, Tommonaro, Giuseppina, Mojović, Miloš, "Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol" in Journal of Liposome Research, 30, no. 3 (2020):218-226, https://doi.org/10.1080/08982104.2019.1625378 . .