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dc.creatorChatzopoulou, Fani
dc.creatorSanmartin, Maite
dc.creatorMellidou, Ifigeneia
dc.creatorPateraki, Irini
dc.creatorKoukounaras, Athanasios
dc.creatorTanou, Georgia
dc.creatorKalamaki, Mary S.
dc.creatorVeljović-Jovanović, Sonja
dc.creatorCvetić-Antić, Tijana
dc.creatorKostas, Stefanos
dc.creatorTsouvaltzis, Pavlos
dc.creatorGrumet, Rebecca
dc.creatorKanellis, Angelos K.
dc.date.accessioned2022-04-05T15:26:54Z
dc.date.available2022-04-05T15:26:54Z
dc.date.issued2020
dc.identifier.issn0981-9428
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/1327
dc.description.abstractAscorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.en
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
dc.relationEuropean Social Fund & Greek National Resources-EPEAEK [IIPYTHAGORAS/319, Synergasia-GR-NUTRITOM/11Syn_3_480]
dc.rightsrestrictedAccess
dc.sourcePlant Physiology and Biochemistry
dc.subjectRipeningen
dc.subjectEthyleneen
dc.subjectCucumis meloen
dc.subjectAscorbic aciden
dc.subjectAscorbate oxidaseen
dc.titleSilencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruiten
dc.typearticle
dc.rights.licenseARR
dc.citation.epage303
dc.citation.other156: 291-303
dc.citation.rankM21
dc.citation.spage291
dc.citation.volume156
dc.identifier.doi10.1016/j.plaphy.2020.08.040
dc.identifier.pmid32987259
dc.identifier.scopus2-s2.0-85091594148
dc.identifier.wos000582643100029
dc.type.versionpublishedVersion


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