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Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit

Authorized Users Only
2020
Authors
Chatzopoulou, Fani
Sanmartin, Maite
Mellidou, Ifigeneia
Pateraki, Irini
Koukounaras, Athanasios
Tanou, Georgia
Kalamaki, Mary S.
Veljović-Jovanović, Sonja
Cvetić-Antić, Tijana
Kostas, Stefanos
Tsouvaltzis, Pavlos
Grumet, Rebecca
Kanellis, Angelos K.
Article (Published version)
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Abstract
Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppress...ed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.

Keywords:
Ripening / Ethylene / Cucumis melo / Ascorbic acid / Ascorbate oxidase
Source:
Plant Physiology and Biochemistry, 2020, 156, 291-303
Publisher:
  • Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
Funding / projects:
  • European Social Fund & Greek National Resources-EPEAEK [IIPYTHAGORAS/319, Synergasia-GR-NUTRITOM/11Syn_3_480]

DOI: 10.1016/j.plaphy.2020.08.040

ISSN: 0981-9428

PubMed: 32987259

WoS: 000582643100029

Scopus: 2-s2.0-85091594148
[ Google Scholar ]
11
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1327
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Chatzopoulou, Fani
AU  - Sanmartin, Maite
AU  - Mellidou, Ifigeneia
AU  - Pateraki, Irini
AU  - Koukounaras, Athanasios
AU  - Tanou, Georgia
AU  - Kalamaki, Mary S.
AU  - Veljović-Jovanović, Sonja
AU  - Cvetić-Antić, Tijana
AU  - Kostas, Stefanos
AU  - Tsouvaltzis, Pavlos
AU  - Grumet, Rebecca
AU  - Kanellis, Angelos K.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1327
AB  - Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit
EP  - 303
SP  - 291
VL  - 156
DO  - 10.1016/j.plaphy.2020.08.040
ER  - 
@article{
author = "Chatzopoulou, Fani and Sanmartin, Maite and Mellidou, Ifigeneia and Pateraki, Irini and Koukounaras, Athanasios and Tanou, Georgia and Kalamaki, Mary S. and Veljović-Jovanović, Sonja and Cvetić-Antić, Tijana and Kostas, Stefanos and Tsouvaltzis, Pavlos and Grumet, Rebecca and Kanellis, Angelos K.",
year = "2020",
abstract = "Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit",
pages = "303-291",
volume = "156",
doi = "10.1016/j.plaphy.2020.08.040"
}
Chatzopoulou, F., Sanmartin, M., Mellidou, I., Pateraki, I., Koukounaras, A., Tanou, G., Kalamaki, M. S., Veljović-Jovanović, S., Cvetić-Antić, T., Kostas, S., Tsouvaltzis, P., Grumet, R.,& Kanellis, A. K.. (2020). Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 156, 291-303.
https://doi.org/10.1016/j.plaphy.2020.08.040
Chatzopoulou F, Sanmartin M, Mellidou I, Pateraki I, Koukounaras A, Tanou G, Kalamaki MS, Veljović-Jovanović S, Cvetić-Antić T, Kostas S, Tsouvaltzis P, Grumet R, Kanellis AK. Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit. in Plant Physiology and Biochemistry. 2020;156:291-303.
doi:10.1016/j.plaphy.2020.08.040 .
Chatzopoulou, Fani, Sanmartin, Maite, Mellidou, Ifigeneia, Pateraki, Irini, Koukounaras, Athanasios, Tanou, Georgia, Kalamaki, Mary S., Veljović-Jovanović, Sonja, Cvetić-Antić, Tijana, Kostas, Stefanos, Tsouvaltzis, Pavlos, Grumet, Rebecca, Kanellis, Angelos K., "Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit" in Plant Physiology and Biochemistry, 156 (2020):291-303,
https://doi.org/10.1016/j.plaphy.2020.08.040 . .

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