Studies on the identification, properties and function of chitin in sponges (Porifera), which are recognized as the first multicellular organisms on Earth, continue to be of fundamental scientific interest. The occurrence of chitin has so far been reported in 21 marine sponge species and only in two inhabiting fresh water. In this study, we present the discovery of alpha-chitin in the endemic demosponge Ochridaspongia rotunda, found in Lake Ohrid, which dates from the Tertiary. The presence of chitin in this species was confirmed using special staining, a chitinase test, FTIR, Raman and NEXAFS spectroscopy, and electrospray ionization mass spectrometry (ESIMS). In contrast to the case of marine sponges, chitin in O. rotunda has been found only within its holdfast, suggesting a role of chitin in the attachment of the sponge to the hard substratum. Isolated fibrous matter strongly resemble the shape and size of the sponge holdfast with membrane-like structure.
Keywords:
Ochridaspongia / Holdfast / Chitin
Source:
International Journal of Biological Macromolecules, 2020, 162, 1187-1194
Publisher:
Elsevier, Amsterdam
Funding / projects:
DFG (Germany)German Research Foundation (DFG) [HE 394/3]
SMWK Project (Germany) [02010311]
Ministry of Science and Higher Education (Poland)Ministry of Science and Higher Education, Poland [0912/SBAD/2006]
RGBL at BESSY II (Berlin, Germany)
Polish National Agency for Academic ExchangePolish National Agency for Academic Exchange (NAWA) [PPN/BEK/2018/1/00071]
TY - JOUR
AU - Talevski, Trajce
AU - Talevska-Leshoska, Aleksandra
AU - Pejoski, Elena
AU - Pejin, Boris
AU - Machalowski, Tomasz
AU - Wysokowski, Marcin
AU - Tsurkan, Mikhail, V
AU - Petrova, Olga
AU - Sivkov, Viktor
AU - Martinović, Rajko
AU - Pantović, Snežana
AU - Khrunyk, Yuliya
AU - Trylis, Volodymyr
AU - Fursov, Andriy
AU - Djurović, Mirko
AU - Jesionowski, Teofil
AU - Ehrlich, Hermann
PY - 2020
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1324
AB - Studies on the identification, properties and function of chitin in sponges (Porifera), which are recognized as the first multicellular organisms on Earth, continue to be of fundamental scientific interest. The occurrence of chitin has so far been reported in 21 marine sponge species and only in two inhabiting fresh water. In this study, we present the discovery of alpha-chitin in the endemic demosponge Ochridaspongia rotunda, found in Lake Ohrid, which dates from the Tertiary. The presence of chitin in this species was confirmed using special staining, a chitinase test, FTIR, Raman and NEXAFS spectroscopy, and electrospray ionization mass spectrometry (ESIMS). In contrast to the case of marine sponges, chitin in O. rotunda has been found only within its holdfast, suggesting a role of chitin in the attachment of the sponge to the hard substratum. Isolated fibrous matter strongly resemble the shape and size of the sponge holdfast with membrane-like structure.
PB - Elsevier, Amsterdam
T2 - International Journal of Biological Macromolecules
T1 - Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937)
EP - 1194
SP - 1187
VL - 162
DO - 10.1016/j.ijbiomac.2020.06.247
ER -
@article{
author = "Talevski, Trajce and Talevska-Leshoska, Aleksandra and Pejoski, Elena and Pejin, Boris and Machalowski, Tomasz and Wysokowski, Marcin and Tsurkan, Mikhail, V and Petrova, Olga and Sivkov, Viktor and Martinović, Rajko and Pantović, Snežana and Khrunyk, Yuliya and Trylis, Volodymyr and Fursov, Andriy and Djurović, Mirko and Jesionowski, Teofil and Ehrlich, Hermann",
year = "2020",
abstract = "Studies on the identification, properties and function of chitin in sponges (Porifera), which are recognized as the first multicellular organisms on Earth, continue to be of fundamental scientific interest. The occurrence of chitin has so far been reported in 21 marine sponge species and only in two inhabiting fresh water. In this study, we present the discovery of alpha-chitin in the endemic demosponge Ochridaspongia rotunda, found in Lake Ohrid, which dates from the Tertiary. The presence of chitin in this species was confirmed using special staining, a chitinase test, FTIR, Raman and NEXAFS spectroscopy, and electrospray ionization mass spectrometry (ESIMS). In contrast to the case of marine sponges, chitin in O. rotunda has been found only within its holdfast, suggesting a role of chitin in the attachment of the sponge to the hard substratum. Isolated fibrous matter strongly resemble the shape and size of the sponge holdfast with membrane-like structure.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937)",
pages = "1194-1187",
volume = "162",
doi = "10.1016/j.ijbiomac.2020.06.247"
}
Talevski, T., Talevska-Leshoska, A., Pejoski, E., Pejin, B., Machalowski, T., Wysokowski, M., Tsurkan, M. V., Petrova, O., Sivkov, V., Martinović, R., Pantović, S., Khrunyk, Y., Trylis, V., Fursov, A., Djurović, M., Jesionowski, T.,& Ehrlich, H.. (2020). Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937). in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 162, 1187-1194.
https://doi.org/10.1016/j.ijbiomac.2020.06.247
Talevski T, Talevska-Leshoska A, Pejoski E, Pejin B, Machalowski T, Wysokowski M, Tsurkan MV, Petrova O, Sivkov V, Martinović R, Pantović S, Khrunyk Y, Trylis V, Fursov A, Djurović M, Jesionowski T, Ehrlich H. Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937). in International Journal of Biological Macromolecules. 2020;162:1187-1194.
doi:10.1016/j.ijbiomac.2020.06.247 .
Talevski, Trajce, Talevska-Leshoska, Aleksandra, Pejoski, Elena, Pejin, Boris, Machalowski, Tomasz, Wysokowski, Marcin, Tsurkan, Mikhail, V, Petrova, Olga, Sivkov, Viktor, Martinović, Rajko, Pantović, Snežana, Khrunyk, Yuliya, Trylis, Volodymyr, Fursov, Andriy, Djurović, Mirko, Jesionowski, Teofil, Ehrlich, Hermann, "Identification and first insights into the structure of chitin from the endemic freshwater demosponge Ochridaspongia rotunda (Arndt, 1937)" in International Journal of Biological Macromolecules, 162 (2020):1187-1194,
https://doi.org/10.1016/j.ijbiomac.2020.06.247 . .
