An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield.
TY - JOUR
AU - Maksimović, Vuk
AU - Mojović, Miloš
AU - Neumann, G
AU - Vučinić, Željko
PY - 2005
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/130
AB - An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield.
PB - New York Acad Sciences, New York
T2 - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1 - Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction
EP - 465
SP - 461
VL - 1048
DO - 10.1196/annals.1342.067
ER -
@article{
author = "Maksimović, Vuk and Mojović, Miloš and Neumann, G and Vučinić, Željko",
year = "2005",
abstract = "An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction",
pages = "465-461",
volume = "1048",
doi = "10.1196/annals.1342.067"
}
Maksimović, V., Mojović, M., Neumann, G.,& Vučinić, Ž.. (2005). Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 461-465.
https://doi.org/10.1196/annals.1342.067
Maksimović V, Mojović M, Neumann G, Vučinić Ž. Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:461-465.
doi:10.1196/annals.1342.067 .
Maksimović, Vuk, Mojović, Miloš, Neumann, G, Vučinić, Željko, "Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):461-465,
https://doi.org/10.1196/annals.1342.067 . .
