RIMSI - Repository of Institute for Multidisciplinary Research
University of Belgrade - Institute for Multidisciplinary Research
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIMSI
  • Institut za multidisciplinarna istraživanja
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   RIMSI
  • Institut za multidisciplinarna istraživanja
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content

Authorized Users Only
2020
Authors
Radotić, Ksenija
Melo, Thor Bernt
Article (Published version)
Metadata
Show full item record
Abstract
For investigations of ongoing processes in plants, such as photosynthesis in conifer leaves, nondestructive and noninvasive measuring techniques are needed. In this paper, a novel approach has been developed for the measurement of chloroplasts' numbers and pigment contents in conifer leaves based on the measurements of leaf absorption spectra using optical fibers and an array spectrophotometer. To eliminate the effect of scattering on the measured absorption spectra, a strategy has been applied taking advantage of the combined use of thin optical fibers normal to the needle's longitudinal axis and the phenomenon that scattering is largest in the forward direction. The optical path in the leaf is nearly the distance between the fiber tips; thus, we were able to obtain the absorption spectrum of the pigments in situ. A effect of the measured absorption spectra, occurring due to the organization of pigments in the leaf and interaction between light and leaf interior, can be accounted for ...by using the so-called Duysens transformation. Using this transformation, pigment contents and the relative number of chloroplasts can be obtained from the measured absorption spectra. We applied the method to observe pigment concentrations in different stages of the greening process in the leaves of two conifer species, Taxus baccata and Picea abies. The presented method may be used to estimate changes in chloroplast number and pigment content during various phases of greening of a species and to observe differences among various species.

Keywords:
Taxus baccata and Norwegian spruce / Relative chloroplasts' number / Pigment content / Optical fibers / Leaf absorption spectra / Duysens transformation
Source:
Journal of Biological Physics, 2020, 46, 1, 33-43
Publisher:
  • Springer, Dordrecht
Funding / projects:
  • Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)

DOI: 10.1007/s10867-020-09539-4

ISSN: 0092-0606

PubMed: 31950327

WoS: 000507706000001

Scopus: 2-s2.0-85078003213
[ Google Scholar ]
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1298
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melo, Thor Bernt
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1298
AB  - For investigations of ongoing processes in plants, such as photosynthesis in conifer leaves, nondestructive and noninvasive measuring techniques are needed. In this paper, a novel approach has been developed for the measurement of chloroplasts' numbers and pigment contents in conifer leaves based on the measurements of leaf absorption spectra using optical fibers and an array spectrophotometer. To eliminate the effect of scattering on the measured absorption spectra, a strategy has been applied taking advantage of the combined use of thin optical fibers normal to the needle's longitudinal axis and the phenomenon that scattering is largest in the forward direction. The optical path in the leaf is nearly the distance between the fiber tips; thus, we were able to obtain the absorption spectrum of the pigments in situ. A effect of the measured absorption spectra, occurring due to the organization of pigments in the leaf and interaction between light and leaf interior, can be accounted for by using the so-called Duysens transformation. Using this transformation, pigment contents and the relative number of chloroplasts can be obtained from the measured absorption spectra. We applied the method to observe pigment concentrations in different stages of the greening process in the leaves of two conifer species, Taxus baccata and Picea abies. The presented method may be used to estimate changes in chloroplast number and pigment content during various phases of greening of a species and to observe differences among various species.
PB  - Springer, Dordrecht
T2  - Journal of Biological Physics
T1  - Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content
EP  - 43
IS  - 1
SP  - 33
VL  - 46
DO  - 10.1007/s10867-020-09539-4
ER  - 
@article{
author = "Radotić, Ksenija and Melo, Thor Bernt",
year = "2020",
abstract = "For investigations of ongoing processes in plants, such as photosynthesis in conifer leaves, nondestructive and noninvasive measuring techniques are needed. In this paper, a novel approach has been developed for the measurement of chloroplasts' numbers and pigment contents in conifer leaves based on the measurements of leaf absorption spectra using optical fibers and an array spectrophotometer. To eliminate the effect of scattering on the measured absorption spectra, a strategy has been applied taking advantage of the combined use of thin optical fibers normal to the needle's longitudinal axis and the phenomenon that scattering is largest in the forward direction. The optical path in the leaf is nearly the distance between the fiber tips; thus, we were able to obtain the absorption spectrum of the pigments in situ. A effect of the measured absorption spectra, occurring due to the organization of pigments in the leaf and interaction between light and leaf interior, can be accounted for by using the so-called Duysens transformation. Using this transformation, pigment contents and the relative number of chloroplasts can be obtained from the measured absorption spectra. We applied the method to observe pigment concentrations in different stages of the greening process in the leaves of two conifer species, Taxus baccata and Picea abies. The presented method may be used to estimate changes in chloroplast number and pigment content during various phases of greening of a species and to observe differences among various species.",
publisher = "Springer, Dordrecht",
journal = "Journal of Biological Physics",
title = "Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content",
pages = "43-33",
number = "1",
volume = "46",
doi = "10.1007/s10867-020-09539-4"
}
Radotić, K.,& Melo, T. B.. (2020). Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content. in Journal of Biological Physics
Springer, Dordrecht., 46(1), 33-43.
https://doi.org/10.1007/s10867-020-09539-4
Radotić K, Melo TB. Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content. in Journal of Biological Physics. 2020;46(1):33-43.
doi:10.1007/s10867-020-09539-4 .
Radotić, Ksenija, Melo, Thor Bernt, "Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content" in Journal of Biological Physics, 46, no. 1 (2020):33-43,
https://doi.org/10.1007/s10867-020-09539-4 . .

DSpace software copyright © 2002-2015  DuraSpace
About RIMSI | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIMSI | Send Feedback

OpenAIRERCUB