Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution

2019
Аутори
Nikolic, Dragana B.
Nesic, Sofija
Bosnic, Dragana

Kravljanac Kostić, Ljiljana

Nikolic, Miroslav

Samardžić, Jelena T.

Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived pl...ants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
Кључне речи:
Strategy II Fe acquisition / silicon / microelement accumulation / iron deficiency / gene expression / barley (Hordeum vulgare)Извор:
Frontiers in Plant Science, 2019, 10Издавач:
- Frontiers Media Sa, Lausanne
Финансирање / пројекти:
- Молекуларни механизми одговора биљака на абиотички стрес-улога транскрипционих фактора и малих РНК и анализа генетичког диверзитета биљних култура од интереса за пољопривреду и биотехнологију (RS-173005)
- Минерални стрес и адаптације биљака на маргиналним пољопривредним земљиштима (RS-173028)
DOI: 10.3389/fpls.2019.00416
ISSN: 1664-462X
PubMed: 31024590
WoS: 000463470300001
Scopus: 2-s2.0-85064227790
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Nikolic, Dragana B. AU - Nesic, Sofija AU - Bosnic, Dragana AU - Kravljanac Kostić, Ljiljana AU - Nikolic, Miroslav AU - Samardžić, Jelena T. PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1284 AB - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Plant Science T1 - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution VL - 10 DO - 10.3389/fpls.2019.00416 ER -
@article{ author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kravljanac Kostić, Ljiljana and Nikolic, Miroslav and Samardžić, Jelena T.", year = "2019", abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Plant Science", title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution", volume = "10", doi = "10.3389/fpls.2019.00416" }
Nikolic, D. B., Nesic, S., Bosnic, D., Kravljanac Kostić, L., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science Frontiers Media Sa, Lausanne., 10. https://doi.org/10.3389/fpls.2019.00416
Nikolic DB, Nesic S, Bosnic D, Kravljanac Kostić L, Nikolic M, Samardžić JT. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10. doi:10.3389/fpls.2019.00416 .
Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kravljanac Kostić, Ljiljana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019), https://doi.org/10.3389/fpls.2019.00416 . .