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P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism
Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply... of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.
TY - JOUR
AU - Spasojević, Ivan
AU - Zakrzewska, Joanna
AU - Bačić, Goran
PY - 2005
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/124
AB - Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.
PB - New York Acad Sciences, New York
T2 - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1 - P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism
EP - 320
SP - 311
VL - 1048
DO - 10.1196/annals.1342.028
ER -
@article{
author = "Spasojević, Ivan and Zakrzewska, Joanna and Bačić, Goran",
year = "2005",
abstract = "Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism",
pages = "320-311",
volume = "1048",
doi = "10.1196/annals.1342.028"
}
Spasojević, I., Zakrzewska, J.,& Bačić, G.. (2005). P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 311-320.
https://doi.org/10.1196/annals.1342.028
Spasojević I, Zakrzewska J, Bačić G. P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:311-320.
doi:10.1196/annals.1342.028 .
Spasojević, Ivan, Zakrzewska, Joanna, Bačić, Goran, "P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):311-320,
https://doi.org/10.1196/annals.1342.028 . .
