Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems
Само за регистроване кориснике
2005
Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.
Кључне речи:
hydrogen atom / EPR spectroscopy / spin trap / Fenton reactionИзвор:
Journal of Chemical Information and Modeling, 2005, 45, 6, 1716-1718Издавач:
- Amer Chemical Soc, Washington
DOI: 10.1021/ci050173d
ISSN: 1549-9596
PubMed: 16309278
WoS: 000233689400036
Scopus: 2-s2.0-28944439903
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Mojović, Miloš AU - Spasojević, Ivan AU - Bačić, Goran PY - 2005 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/122 AB - We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems. PB - Amer Chemical Soc, Washington T2 - Journal of Chemical Information and Modeling T1 - Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems EP - 1718 IS - 6 SP - 1716 VL - 45 DO - 10.1021/ci050173d ER -
@article{ author = "Mojović, Miloš and Spasojević, Ivan and Bačić, Goran", year = "2005", abstract = "We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.", publisher = "Amer Chemical Soc, Washington", journal = "Journal of Chemical Information and Modeling", title = "Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems", pages = "1718-1716", number = "6", volume = "45", doi = "10.1021/ci050173d" }
Mojović, M., Spasojević, I.,& Bačić, G.. (2005). Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems. in Journal of Chemical Information and Modeling Amer Chemical Soc, Washington., 45(6), 1716-1718. https://doi.org/10.1021/ci050173d
Mojović M, Spasojević I, Bačić G. Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems. in Journal of Chemical Information and Modeling. 2005;45(6):1716-1718. doi:10.1021/ci050173d .
Mojović, Miloš, Spasojević, Ivan, Bačić, Goran, "Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems" in Journal of Chemical Information and Modeling, 45, no. 6 (2005):1716-1718, https://doi.org/10.1021/ci050173d . .