We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.
TY - JOUR
AU - Mojović, Miloš
AU - Spasojević, Ivan
AU - Bačić, Goran
PY - 2005
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/122
AB - We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.
PB - Amer Chemical Soc, Washington
T2 - Journal of Chemical Information and Modeling
T1 - Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems
EP - 1718
IS - 6
SP - 1716
VL - 45
DO - 10.1021/ci050173d
ER -
@article{
author = "Mojović, Miloš and Spasojević, Ivan and Bačić, Goran",
year = "2005",
abstract = "We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Chemical Information and Modeling",
title = "Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems",
pages = "1718-1716",
number = "6",
volume = "45",
doi = "10.1021/ci050173d"
}
Mojović, M., Spasojević, I.,& Bačić, G.. (2005). Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems. in Journal of Chemical Information and Modeling
Amer Chemical Soc, Washington., 45(6), 1716-1718.
https://doi.org/10.1021/ci050173d
Mojović M, Spasojević I, Bačić G. Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems. in Journal of Chemical Information and Modeling. 2005;45(6):1716-1718.
doi:10.1021/ci050173d .
Mojović, Miloš, Spasojević, Ivan, Bačić, Goran, "Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems" in Journal of Chemical Information and Modeling, 45, no. 6 (2005):1716-1718,
https://doi.org/10.1021/ci050173d . .
