Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip
2019
Аутори
Popović, TatjanaJelušić, Aleksandra
Dimkić, Ivica
Stanković, Slaviša
Postic, Dobrivoj
Aleksic, Goran
Veljović-Jovanović, Sonja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG(5), REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induc...tion and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.
Кључне речи:
Pseudomonas syringae pv. coriandricola / bacterial leaf spot / pathogenicity / biochemical analysisИзвор:
Plant Disease, 2019, 103, 12, 3072-3082Издавач:
- Amer Phytopathological Soc, St Paul
Финансирање / пројекти:
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
- Молекуларна карактеризација бактерија из родова Bacillus и Pseudomonas као потенцијалних агенаса за биолошку контролу (RS-MESTD-Basic Research (BR or ON)-173026)
DOI: 10.1094/PDIS-03-19-0674-RE
ISSN: 0191-2917
PubMed: 31596690
WoS: 000499765800011
Scopus: 2-s2.0-85075813149
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Popović, Tatjana AU - Jelušić, Aleksandra AU - Dimkić, Ivica AU - Stanković, Slaviša AU - Postic, Dobrivoj AU - Aleksic, Goran AU - Veljović-Jovanović, Sonja PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1208 AB - Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG(5), REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip. PB - Amer Phytopathological Soc, St Paul T2 - Plant Disease T1 - Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip EP - 3082 IS - 12 SP - 3072 VL - 103 DO - 10.1094/PDIS-03-19-0674-RE ER -
@article{ author = "Popović, Tatjana and Jelušić, Aleksandra and Dimkić, Ivica and Stanković, Slaviša and Postic, Dobrivoj and Aleksic, Goran and Veljović-Jovanović, Sonja", year = "2019", abstract = "Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG(5), REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.", publisher = "Amer Phytopathological Soc, St Paul", journal = "Plant Disease", title = "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip", pages = "3082-3072", number = "12", volume = "103", doi = "10.1094/PDIS-03-19-0674-RE" }
Popović, T., Jelušić, A., Dimkić, I., Stanković, S., Postic, D., Aleksic, G.,& Veljović-Jovanović, S.. (2019). Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease Amer Phytopathological Soc, St Paul., 103(12), 3072-3082. https://doi.org/10.1094/PDIS-03-19-0674-RE
Popović T, Jelušić A, Dimkić I, Stanković S, Postic D, Aleksic G, Veljović-Jovanović S. Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease. 2019;103(12):3072-3082. doi:10.1094/PDIS-03-19-0674-RE .
Popović, Tatjana, Jelušić, Aleksandra, Dimkić, Ivica, Stanković, Slaviša, Postic, Dobrivoj, Aleksic, Goran, Veljović-Jovanović, Sonja, "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip" in Plant Disease, 103, no. 12 (2019):3072-3082, https://doi.org/10.1094/PDIS-03-19-0674-RE . .