Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract

Abstract

This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of alpha and ss glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 +/- 0.59 mu g/mL) and high potential for reduction of Fe3+ ions (174.11 +/- 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 mu g), the same extract exhibited a moderate anti-AChE activity (10 mu g) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 +/- 0.31 mu g/mL, 197.68 +/- 9.47 mg eq. AA/g d.w. and 0.1 mu g, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully addressing both oxidative stress and lack of acetylcholine.