Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance
Abstract
Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based o...n a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction.
Keywords:
Wheat / TaALMT1 / malate / aluminium toleranceSource:
Journal of Soil Science and Plant Nutrition, 2018, 18, 1, 90-99Publisher:
- Sociedad Chilena de la Ciencia del Suelo
Funding / projects:
DOI: 10.4067/S0718-95162018005000402
ISSN: 0718-9516
WoS: 000432489200007
Scopus: 2-s2.0-85047016582
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Savić, J. AU - Stević, N. AU - Maksimović, Vuk AU - Samardžić, Jelena T. AU - Nikolic, D.B. AU - Nikolic, M. PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1179 AB - Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based on a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction. PB - Sociedad Chilena de la Ciencia del Suelo T2 - Journal of Soil Science and Plant Nutrition T1 - Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance EP - 99 IS - 1 SP - 90 VL - 18 DO - 10.4067/S0718-95162018005000402 ER -
@article{ author = "Savić, J. and Stević, N. and Maksimović, Vuk and Samardžić, Jelena T. and Nikolic, D.B. and Nikolic, M.", year = "2018", abstract = "Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based on a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction.", publisher = "Sociedad Chilena de la Ciencia del Suelo", journal = "Journal of Soil Science and Plant Nutrition", title = "Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance", pages = "99-90", number = "1", volume = "18", doi = "10.4067/S0718-95162018005000402" }
Savić, J., Stević, N., Maksimović, V., Samardžić, J. T., Nikolic, D.B.,& Nikolic, M.. (2018). Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance. in Journal of Soil Science and Plant Nutrition Sociedad Chilena de la Ciencia del Suelo., 18(1), 90-99. https://doi.org/10.4067/S0718-95162018005000402
Savić J, Stević N, Maksimović V, Samardžić JT, Nikolic D, Nikolic M. Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance. in Journal of Soil Science and Plant Nutrition. 2018;18(1):90-99. doi:10.4067/S0718-95162018005000402 .
Savić, J., Stević, N., Maksimović, Vuk, Samardžić, Jelena T., Nikolic, D.B., Nikolic, M., "Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance" in Journal of Soil Science and Plant Nutrition, 18, no. 1 (2018):90-99, https://doi.org/10.4067/S0718-95162018005000402 . .