Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.
TY - CHAP
AU - Bogdanović Pristov, Jelena
AU - Spasojević, Ivan
PY - 2018
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1176
AB - Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.
PB - Humana Press Inc.
T2 - Methods in Molecular Biology
T1 - A one-step staining protocol for in-gel fluorescent visualization of proteins
EP - 158
SP - 149
VL - 1853
DO - 10.1007/978-1-4939-8745-0_18
ER -
@inbook{
author = "Bogdanović Pristov, Jelena and Spasojević, Ivan",
year = "2018",
abstract = "Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.",
publisher = "Humana Press Inc.",
journal = "Methods in Molecular Biology",
booktitle = "A one-step staining protocol for in-gel fluorescent visualization of proteins",
pages = "158-149",
volume = "1853",
doi = "10.1007/978-1-4939-8745-0_18"
}
Bogdanović Pristov, J.,& Spasojević, I.. (2018). A one-step staining protocol for in-gel fluorescent visualization of proteins. in Methods in Molecular Biology
Humana Press Inc.., 1853, 149-158.
https://doi.org/10.1007/978-1-4939-8745-0_18
Bogdanović Pristov J, Spasojević I. A one-step staining protocol for in-gel fluorescent visualization of proteins. in Methods in Molecular Biology. 2018;1853:149-158.
doi:10.1007/978-1-4939-8745-0_18 .
Bogdanović Pristov, Jelena, Spasojević, Ivan, "A one-step staining protocol for in-gel fluorescent visualization of proteins" in Methods in Molecular Biology, 1853 (2018):149-158,
https://doi.org/10.1007/978-1-4939-8745-0_18 . .
