In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.
TY - JOUR
AU - Milivojević, S
AU - Mitrović, Aleksandra
AU - Culafic, L
PY - 2005
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/117
AB - In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.
PB - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2 - Biologia Plantarum
T1 - Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro
EP - 39
IS - 1
SP - 35
VL - 49
DO - 10.1007/s00000-005-5039-5
ER -
@article{
author = "Milivojević, S and Mitrović, Aleksandra and Culafic, L",
year = "2005",
abstract = "In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro",
pages = "39-35",
number = "1",
volume = "49",
doi = "10.1007/s00000-005-5039-5"
}
Milivojević, S., Mitrović, A.,& Culafic, L.. (2005). Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 49(1), 35-39.
https://doi.org/10.1007/s00000-005-5039-5
Milivojević S, Mitrović A, Culafic L. Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro. in Biologia Plantarum. 2005;49(1):35-39.
doi:10.1007/s00000-005-5039-5 .
Milivojević, S, Mitrović, Aleksandra, Culafic, L, "Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro" in Biologia Plantarum, 49, no. 1 (2005):35-39,
https://doi.org/10.1007/s00000-005-5039-5 . .
