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Anti-cancer effects of wedelolactone: interactions with copper and subcellular localization

Authorized Users Only
2018
Authors
Kucirkova, Tereza
Stiborek, Marek
Ducka, Monika
Navratilova, Jarmila
Bogdanović Pristov, Jelena
Popovic-Bijelic, Ana
Vojvodić, Snežana
Preisler, Jan
Kanicky, Viktor
Smarda, Jan
Spasojević, Ivan
Benes, Petr
Article (Published version)
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Abstract
Wedelactone (WL), a plant polyphenolic derivative of coumestan, represents a promising anti-cancer agent. The underlying mechanisms of its action are not fully understood and appear to involve interplay with copper ions. Herein, we examined coordination and redox interactions of WL with Cu2+ in phosphate buffer (pH 7), and in two breast cancer cell lines. EPR, UV-Vis and fluorescence spectroscopy showed that WL and Cu2+ build a coordination complex with 2:1 stoichiometry and distorted tetrahedral geometry. WL showed strong fluorescence that was quenched by Cu2+. The sequestration of the intracellular copper pool with neocuproine led to a significant drop in the cytotoxic effects of WL, whereas the co-application of Cu2+ and WL and the formation of an extracellular complex suppressed both the cytotoxic effects of WL and copper loading. Fluorescence microscopy showed that WL is mainly localized in the cytosol and significantly less in the nuclei. WL fluorescence was stronger in cells pre...treated with neocuproine, implying that the complex of WL and Cu2+ is formed inside the cells. WL caused a two-fold increase in the lysosomal level of copper as well as copper-dependent lysosome membrane permeabilization. On the other hand, the protective effects of overexpression of thioredoxin 1 imply that WL exerts the main oxidative impact inside the nucleus. The interactions of WL with copper may be essential for therapeutic performance and selectivity against cancer cells, taking into account that a number of cancer types, including breast cancer, exhibit increased intratumoral copper levels or altered copper distribution.

Source:
Metallomics, 2018, 10, 10, 1524-1531
Publisher:
  • Royal Soc Chemistry, Cambridge
Funding / projects:
  • Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
  • National Program of Sustainability II (MEYS Czech Republic) [LQ1605]
  • Czech Science FoundationGrant Agency of the Czech Republic [18-16583S]
  • Grant Agency of the Masaryk University [MUNI/G/0974/2016, MUNI/A/0824/2017]

DOI: 10.1039/c8mt00191j

ISSN: 1756-5901

PubMed: 30238942

WoS: 000448343400014

Scopus: 2-s2.0-85055078004
[ Google Scholar ]
5
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1156
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Kucirkova, Tereza
AU  - Stiborek, Marek
AU  - Ducka, Monika
AU  - Navratilova, Jarmila
AU  - Bogdanović Pristov, Jelena
AU  - Popovic-Bijelic, Ana
AU  - Vojvodić, Snežana
AU  - Preisler, Jan
AU  - Kanicky, Viktor
AU  - Smarda, Jan
AU  - Spasojević, Ivan
AU  - Benes, Petr
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1156
AB  - Wedelactone (WL), a plant polyphenolic derivative of coumestan, represents a promising anti-cancer agent. The underlying mechanisms of its action are not fully understood and appear to involve interplay with copper ions. Herein, we examined coordination and redox interactions of WL with Cu2+ in phosphate buffer (pH 7), and in two breast cancer cell lines. EPR, UV-Vis and fluorescence spectroscopy showed that WL and Cu2+ build a coordination complex with 2:1 stoichiometry and distorted tetrahedral geometry. WL showed strong fluorescence that was quenched by Cu2+. The sequestration of the intracellular copper pool with neocuproine led to a significant drop in the cytotoxic effects of WL, whereas the co-application of Cu2+ and WL and the formation of an extracellular complex suppressed both the cytotoxic effects of WL and copper loading. Fluorescence microscopy showed that WL is mainly localized in the cytosol and significantly less in the nuclei. WL fluorescence was stronger in cells pretreated with neocuproine, implying that the complex of WL and Cu2+ is formed inside the cells. WL caused a two-fold increase in the lysosomal level of copper as well as copper-dependent lysosome membrane permeabilization. On the other hand, the protective effects of overexpression of thioredoxin 1 imply that WL exerts the main oxidative impact inside the nucleus. The interactions of WL with copper may be essential for therapeutic performance and selectivity against cancer cells, taking into account that a number of cancer types, including breast cancer, exhibit increased intratumoral copper levels or altered copper distribution.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Anti-cancer effects of wedelolactone: interactions with copper and subcellular localization
EP  - 1531
IS  - 10
SP  - 1524
VL  - 10
DO  - 10.1039/c8mt00191j
ER  - 
@article{
author = "Kucirkova, Tereza and Stiborek, Marek and Ducka, Monika and Navratilova, Jarmila and Bogdanović Pristov, Jelena and Popovic-Bijelic, Ana and Vojvodić, Snežana and Preisler, Jan and Kanicky, Viktor and Smarda, Jan and Spasojević, Ivan and Benes, Petr",
year = "2018",
abstract = "Wedelactone (WL), a plant polyphenolic derivative of coumestan, represents a promising anti-cancer agent. The underlying mechanisms of its action are not fully understood and appear to involve interplay with copper ions. Herein, we examined coordination and redox interactions of WL with Cu2+ in phosphate buffer (pH 7), and in two breast cancer cell lines. EPR, UV-Vis and fluorescence spectroscopy showed that WL and Cu2+ build a coordination complex with 2:1 stoichiometry and distorted tetrahedral geometry. WL showed strong fluorescence that was quenched by Cu2+. The sequestration of the intracellular copper pool with neocuproine led to a significant drop in the cytotoxic effects of WL, whereas the co-application of Cu2+ and WL and the formation of an extracellular complex suppressed both the cytotoxic effects of WL and copper loading. Fluorescence microscopy showed that WL is mainly localized in the cytosol and significantly less in the nuclei. WL fluorescence was stronger in cells pretreated with neocuproine, implying that the complex of WL and Cu2+ is formed inside the cells. WL caused a two-fold increase in the lysosomal level of copper as well as copper-dependent lysosome membrane permeabilization. On the other hand, the protective effects of overexpression of thioredoxin 1 imply that WL exerts the main oxidative impact inside the nucleus. The interactions of WL with copper may be essential for therapeutic performance and selectivity against cancer cells, taking into account that a number of cancer types, including breast cancer, exhibit increased intratumoral copper levels or altered copper distribution.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Anti-cancer effects of wedelolactone: interactions with copper and subcellular localization",
pages = "1531-1524",
number = "10",
volume = "10",
doi = "10.1039/c8mt00191j"
}
Kucirkova, T., Stiborek, M., Ducka, M., Navratilova, J., Bogdanović Pristov, J., Popovic-Bijelic, A., Vojvodić, S., Preisler, J., Kanicky, V., Smarda, J., Spasojević, I.,& Benes, P.. (2018). Anti-cancer effects of wedelolactone: interactions with copper and subcellular localization. in Metallomics
Royal Soc Chemistry, Cambridge., 10(10), 1524-1531.
https://doi.org/10.1039/c8mt00191j
Kucirkova T, Stiborek M, Ducka M, Navratilova J, Bogdanović Pristov J, Popovic-Bijelic A, Vojvodić S, Preisler J, Kanicky V, Smarda J, Spasojević I, Benes P. Anti-cancer effects of wedelolactone: interactions with copper and subcellular localization. in Metallomics. 2018;10(10):1524-1531.
doi:10.1039/c8mt00191j .
Kucirkova, Tereza, Stiborek, Marek, Ducka, Monika, Navratilova, Jarmila, Bogdanović Pristov, Jelena, Popovic-Bijelic, Ana, Vojvodić, Snežana, Preisler, Jan, Kanicky, Viktor, Smarda, Jan, Spasojević, Ivan, Benes, Petr, "Anti-cancer effects of wedelolactone: interactions with copper and subcellular localization" in Metallomics, 10, no. 10 (2018):1524-1531,
https://doi.org/10.1039/c8mt00191j . .

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