Effects of vanadate on the mycelium of edible fungus Coprinus comatus
Само за регистроване кориснике
2018
Аутори
Žižić, MilanZakrzewska, Joanna
Tesanović, Kristina
Bosković, Eleonora
Nesović, Milica
Karaman, Maja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but van...adyl uptake is significantly less comparing to vanadate.
Кључне речи:
Vanadium / SEM / Phosphate metabolism / NMR / EPR / Coprinus comatusИзвор:
Journal of Trace Elements in Medicine and Biology, 2018, 50, 320-326Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
- Биолошки активни природни производи као потенцијални извори нових лекова и дијететских суплемената (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.1016/j.jtemb.2018.07.017
ISSN: 0946-672X
PubMed: 30262298
WoS: 000448633900043
Scopus: 2-s2.0-85050675386
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Žižić, Milan AU - Zakrzewska, Joanna AU - Tesanović, Kristina AU - Bosković, Eleonora AU - Nesović, Milica AU - Karaman, Maja PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1119 AB - Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate. PB - Elsevier Gmbh, Munich T2 - Journal of Trace Elements in Medicine and Biology T1 - Effects of vanadate on the mycelium of edible fungus Coprinus comatus EP - 326 SP - 320 VL - 50 DO - 10.1016/j.jtemb.2018.07.017 ER -
@article{ author = "Žižić, Milan and Zakrzewska, Joanna and Tesanović, Kristina and Bosković, Eleonora and Nesović, Milica and Karaman, Maja", year = "2018", abstract = "Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.", publisher = "Elsevier Gmbh, Munich", journal = "Journal of Trace Elements in Medicine and Biology", title = "Effects of vanadate on the mycelium of edible fungus Coprinus comatus", pages = "326-320", volume = "50", doi = "10.1016/j.jtemb.2018.07.017" }
Žižić, M., Zakrzewska, J., Tesanović, K., Bosković, E., Nesović, M.,& Karaman, M.. (2018). Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology Elsevier Gmbh, Munich., 50, 320-326. https://doi.org/10.1016/j.jtemb.2018.07.017
Žižić M, Zakrzewska J, Tesanović K, Bosković E, Nesović M, Karaman M. Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology. 2018;50:320-326. doi:10.1016/j.jtemb.2018.07.017 .
Žižić, Milan, Zakrzewska, Joanna, Tesanović, Kristina, Bosković, Eleonora, Nesović, Milica, Karaman, Maja, "Effects of vanadate on the mycelium of edible fungus Coprinus comatus" in Journal of Trace Elements in Medicine and Biology, 50 (2018):320-326, https://doi.org/10.1016/j.jtemb.2018.07.017 . .