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Enzymatic Profile of 'Willamette' Raspberry Leaf and Fruit Affected by Prohexadione-Ca and Young Canes Removal Treatments

Authorized Users Only
2017
Authors
Dragišić Maksimović, Jelena
Poledica, Milena M
Radivojević, Dragan
Milivojević, Jasminka M
Article (Published version)
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Abstract
The influence of growth regulator prohexadione-Ca (ProCa) concurrently with young canes removal on the modification of photosynthetic pigments content and antioxidant enzymes (peroxidase, POD; catalase, CAT; polyphenol oxidase, PPO; superoxide dismutase, SOD) activities in leaves and fruits of raspberry (Rubus idaeus L.) cultivar 'Willamette' was studied. ProCa increased while canes removal decreased chlorophylls and carotenoids content compared to control. POD, CAT, and PPO activities in leaves after removal of young canes were higher compared to control (2-4 times) which was visually confirmed for POD by isoelectrofocusing. Removal of young canes slithly increased, while ProCa significantly enhanced SOD activity in leaves compared to control (475.10 and 218.38 nkat mg(-1) prot, respectively). Pattern of SOD activity in fruit was similar as in leaf with substantial increase compared to control (about 15 times). Combination of implemented measures increased activity of all enzymes in t...he leaves and fruits. Our study could provide a better knowledge of the ProCa and canes removal influences on the action of enzymes in order to regulate their activities in fruit products.

Keywords:
superoxide dismutase / Rubus idaeus L. / polyphenol oxidase / peroxidase / isoelectric focusing / chlorophyll / catalase / carotenoids
Source:
Journal of Agricultural and Food Chemistry, 2017, 65, 24, 5034-5040
Publisher:
  • Amer Chemical Soc, Washington
Funding / projects:
  • The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-173040)
  • Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)

DOI: 10.1021/acs.jafc.7b00638

ISSN: 0021-8561

PubMed: 28581737

WoS: 000404090400020

Scopus: 2-s2.0-85021120936
[ Google Scholar ]
5
4
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1099
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Poledica, Milena M
AU  - Radivojević, Dragan
AU  - Milivojević, Jasminka M
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1099
AB  - The influence of growth regulator prohexadione-Ca (ProCa) concurrently with young canes removal on the modification of photosynthetic pigments content and antioxidant enzymes (peroxidase, POD; catalase, CAT; polyphenol oxidase, PPO; superoxide dismutase, SOD) activities in leaves and fruits of raspberry (Rubus idaeus L.) cultivar 'Willamette' was studied. ProCa increased while canes removal decreased chlorophylls and carotenoids content compared to control. POD, CAT, and PPO activities in leaves after removal of young canes were higher compared to control (2-4 times) which was visually confirmed for POD by isoelectrofocusing. Removal of young canes slithly increased, while ProCa significantly enhanced SOD activity in leaves compared to control (475.10 and 218.38 nkat mg(-1) prot, respectively). Pattern of SOD activity in fruit was similar as in leaf with substantial increase compared to control (about 15 times). Combination of implemented measures increased activity of all enzymes in the leaves and fruits. Our study could provide a better knowledge of the ProCa and canes removal influences on the action of enzymes in order to regulate their activities in fruit products.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Enzymatic Profile of 'Willamette' Raspberry Leaf and Fruit Affected by Prohexadione-Ca and Young Canes Removal Treatments
EP  - 5040
IS  - 24
SP  - 5034
VL  - 65
DO  - 10.1021/acs.jafc.7b00638
ER  - 
@article{
author = "Dragišić Maksimović, Jelena and Poledica, Milena M and Radivojević, Dragan and Milivojević, Jasminka M",
year = "2017",
abstract = "The influence of growth regulator prohexadione-Ca (ProCa) concurrently with young canes removal on the modification of photosynthetic pigments content and antioxidant enzymes (peroxidase, POD; catalase, CAT; polyphenol oxidase, PPO; superoxide dismutase, SOD) activities in leaves and fruits of raspberry (Rubus idaeus L.) cultivar 'Willamette' was studied. ProCa increased while canes removal decreased chlorophylls and carotenoids content compared to control. POD, CAT, and PPO activities in leaves after removal of young canes were higher compared to control (2-4 times) which was visually confirmed for POD by isoelectrofocusing. Removal of young canes slithly increased, while ProCa significantly enhanced SOD activity in leaves compared to control (475.10 and 218.38 nkat mg(-1) prot, respectively). Pattern of SOD activity in fruit was similar as in leaf with substantial increase compared to control (about 15 times). Combination of implemented measures increased activity of all enzymes in the leaves and fruits. Our study could provide a better knowledge of the ProCa and canes removal influences on the action of enzymes in order to regulate their activities in fruit products.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Enzymatic Profile of 'Willamette' Raspberry Leaf and Fruit Affected by Prohexadione-Ca and Young Canes Removal Treatments",
pages = "5040-5034",
number = "24",
volume = "65",
doi = "10.1021/acs.jafc.7b00638"
}
Dragišić Maksimović, J., Poledica, M. M., Radivojević, D.,& Milivojević, J. M.. (2017). Enzymatic Profile of 'Willamette' Raspberry Leaf and Fruit Affected by Prohexadione-Ca and Young Canes Removal Treatments. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 65(24), 5034-5040.
https://doi.org/10.1021/acs.jafc.7b00638
Dragišić Maksimović J, Poledica MM, Radivojević D, Milivojević JM. Enzymatic Profile of 'Willamette' Raspberry Leaf and Fruit Affected by Prohexadione-Ca and Young Canes Removal Treatments. in Journal of Agricultural and Food Chemistry. 2017;65(24):5034-5040.
doi:10.1021/acs.jafc.7b00638 .
Dragišić Maksimović, Jelena, Poledica, Milena M, Radivojević, Dragan, Milivojević, Jasminka M, "Enzymatic Profile of 'Willamette' Raspberry Leaf and Fruit Affected by Prohexadione-Ca and Young Canes Removal Treatments" in Journal of Agricultural and Food Chemistry, 65, no. 24 (2017):5034-5040,
https://doi.org/10.1021/acs.jafc.7b00638 . .

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