Senescence-related changes in the antioxidant status of ginkgo and birch leaves during autumn yellowing

Abstract

The antioxidant status of birch and ginkgo leaves during autumnal senescence was characterized by the activities of catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and superoxide dismutase (SOD). The contents of leaf H2O2 and ascorbate were used as indicators of oxidative stress. Degradation of chlorophyll (chl) during natural senescence was not accompanied either by an increase of H2O2 or by a decrease of reduced ascorbate. A transient decrease of reduced ascorbate in ginkgo and birch leaves in early senescence was accompanied by CAT inactivation. The activity of ionically-bound PODs was stimulated in late senescence in both species, when more than 30% of chl was degraded. Induction of MnSOD in both species and new isoforms of CuZnSOD in birch in late senescence was accompanied by the disappearance of other CuZnSOD isoforms in birch and FeSOD in ginkgo. The role of antioxidative enzymes in keeping ascorbate reduced and endogenous H2O2 at low levels in senescent leaves of deciduous trees was discussed.