Air pollutant effects on chlorenchyma cell. And chloroplasts of douglas-fir (pseudotsuga menziesii (mire.) Franco) needles

Abstract

This paper presents the results of air-pollutant influence on one-year and two-year old needles of Douglas-fir. The aim of this research was determining of resistance of these species. The analysis was performed on samples collected in a Douglas-fir culture established in polluted area of Kolubara coal basin during re-cultivation process of remained material. Control samples from unpolluted area were collected in Douglas-fir culture near mountain Juhor. There has been examined injuries of chlorenchyma cells, evaluating the degree of damage to the chlorenchyma tissue based on the stage of cell disruption which affected the way of the appearance of tannins in the central vacuole. The obtained results showed that the surface area of damaged chlorenchyma cells was significantly increased in polluted environment. At cellular level, there was no complete destruction of thylakoid system, and negative influence of air-pollutants before all affected changing of chloroplasts form, stroma granulation, increasing of thylakoid area, changing of plastoglobulins and collecting of fat in cytoplasm. This kind of changes in one-year and two-year old needles in samples originated from polluted area of Kolubara coal basin included numerous cells from chlorenchyma tissue. This confirms that Douglas-fir is not resistant on air-pollutants influence in researched area.