Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different... cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.
TY - JOUR
AU - Simonović Radosavljević, Jasna
AU - Bogdanović Pristov, Jelena
AU - Mitrović, Aleksandra
AU - Steinbach, Gabor
AU - Mouille, Gregory
AU - Tufegdžić, Srđan J.
AU - Maksimović, Vuk
AU - Mutavdžić, Dragosav
AU - Janosević, Dusica
AU - Vuković, Marina
AU - Garab, Gyozo
AU - Radotić, Ksenija
PY - 2017
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1057
AB - Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.
PB - Springer, Dordrecht
T2 - Cellulose
T1 - Parenchyma cell wall structure in twining stem of Dioscorea balcanica
EP - 4669
IS - 11
SP - 4653
VL - 24
DO - 10.1007/s10570-017-1460-1
ER -
@article{
author = "Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Mitrović, Aleksandra and Steinbach, Gabor and Mouille, Gregory and Tufegdžić, Srđan J. and Maksimović, Vuk and Mutavdžić, Dragosav and Janosević, Dusica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2017",
abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Parenchyma cell wall structure in twining stem of Dioscorea balcanica",
pages = "4669-4653",
number = "11",
volume = "24",
doi = "10.1007/s10570-017-1460-1"
}
Simonović Radosavljević, J., Bogdanović Pristov, J., Mitrović, A., Steinbach, G., Mouille, G., Tufegdžić, S. J., Maksimović, V., Mutavdžić, D., Janosević, D., Vuković, M., Garab, G.,& Radotić, K.. (2017). Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose
Springer, Dordrecht., 24(11), 4653-4669.
https://doi.org/10.1007/s10570-017-1460-1
Simonović Radosavljević J, Bogdanović Pristov J, Mitrović A, Steinbach G, Mouille G, Tufegdžić SJ, Maksimović V, Mutavdžić D, Janosević D, Vuković M, Garab G, Radotić K. Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose. 2017;24(11):4653-4669.
doi:10.1007/s10570-017-1460-1 .
