Moderate hyperhomocysteinemia induced by short-term dietary methionine overload alters bone microarchitecture and collagen features during growth
Само за регистроване кориснике
2017
Аутори
Milovanović, PetarHrncic, Dragan
Radotić, Ksenija
Stanković, Mira
Mutavdžić, Dragosav
Djonic, Danijela
Rasic-Marković, Aleksandra
Djuric, Dragan
Stanojlović, Olivera
Djuric, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aims: In general, hyperhomocysteinemia is increasingly appreciated as a risk factor for various diseases, including osteoporosis. However, its effects in non-adults remain largely unknown. Our aim was to determine whether dietary-caused increased homocysteine levels have deleterious effects on bone structure during growth. Main methods: We developed a model of moderate hyperhomocysteinemia caused by short-term methionine nutritional overload in growing rats. 30-days-old male Wistar albino rats were randomly assigned to either experimental group subject to a 30-days hypermethionine diet or control group. High-resolution 3D assessment of bone geometry and microarchitecture, as well as fluorescence spectroscopic analysis of bone matrix were performed. Key findings: Short-term moderate hyperhomocysteinemia (similar to 30 mu mol/L) achieved in the study notably affected bone and cartilage characteristics. Parameters of the cortical bone geometry in the experimental group indicated peculiar ...reorganization of the bone cross-section. Trabecular bone microarchitecture was especially sensitive to hyperhomocysteinemia showing clearly negative bone balance in the experimental group (almost 30% reduced bone volume, mainly due to similar to 25% decrease in trabecular number as well as markedly reduced trabecular connections). Fluorescent spectroscopy of bone matrix revealed multiple alterations to collagen spectra due to homocysteine accumulation in bone, indicative of broken collagenous cross-links. Significance: Given that appropriate accrual of bone mass during growth has important effects on the risk of osteoporosis in adulthood, understanding the skeletal effects of dietary-induced hyperhomocysteinemia in nonadults is essential for interpreting its importance as a modifiable risk factor for osteoporosis and improving programs to preserve/re-establish bone health.
Кључне речи:
Tissue microarchitecture / Homocysteine / Fluorescent spectroscopy / Development / Collagen / BoneИзвор:
Life Sciences, 2017, 191, 9-16Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Функционални, функционализовани и усавршени нано материјали (RS-45005)
- Развој анималних модела епилепсије и тестирање конвулзивних и антиконвулзивних супстанци (RS-175032)
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-173017)
DOI: 10.1016/j.lfs.2017.10.008
ISSN: 0024-3205
PubMed: 28987632
WoS: 000414376500002
Scopus: 2-s2.0-85030973456
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Milovanović, Petar AU - Hrncic, Dragan AU - Radotić, Ksenija AU - Stanković, Mira AU - Mutavdžić, Dragosav AU - Djonic, Danijela AU - Rasic-Marković, Aleksandra AU - Djuric, Dragan AU - Stanojlović, Olivera AU - Djuric, Marija PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1039 AB - Aims: In general, hyperhomocysteinemia is increasingly appreciated as a risk factor for various diseases, including osteoporosis. However, its effects in non-adults remain largely unknown. Our aim was to determine whether dietary-caused increased homocysteine levels have deleterious effects on bone structure during growth. Main methods: We developed a model of moderate hyperhomocysteinemia caused by short-term methionine nutritional overload in growing rats. 30-days-old male Wistar albino rats were randomly assigned to either experimental group subject to a 30-days hypermethionine diet or control group. High-resolution 3D assessment of bone geometry and microarchitecture, as well as fluorescence spectroscopic analysis of bone matrix were performed. Key findings: Short-term moderate hyperhomocysteinemia (similar to 30 mu mol/L) achieved in the study notably affected bone and cartilage characteristics. Parameters of the cortical bone geometry in the experimental group indicated peculiar reorganization of the bone cross-section. Trabecular bone microarchitecture was especially sensitive to hyperhomocysteinemia showing clearly negative bone balance in the experimental group (almost 30% reduced bone volume, mainly due to similar to 25% decrease in trabecular number as well as markedly reduced trabecular connections). Fluorescent spectroscopy of bone matrix revealed multiple alterations to collagen spectra due to homocysteine accumulation in bone, indicative of broken collagenous cross-links. Significance: Given that appropriate accrual of bone mass during growth has important effects on the risk of osteoporosis in adulthood, understanding the skeletal effects of dietary-induced hyperhomocysteinemia in nonadults is essential for interpreting its importance as a modifiable risk factor for osteoporosis and improving programs to preserve/re-establish bone health. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Life Sciences T1 - Moderate hyperhomocysteinemia induced by short-term dietary methionine overload alters bone microarchitecture and collagen features during growth EP - 16 SP - 9 VL - 191 DO - 10.1016/j.lfs.2017.10.008 ER -
@article{ author = "Milovanović, Petar and Hrncic, Dragan and Radotić, Ksenija and Stanković, Mira and Mutavdžić, Dragosav and Djonic, Danijela and Rasic-Marković, Aleksandra and Djuric, Dragan and Stanojlović, Olivera and Djuric, Marija", year = "2017", abstract = "Aims: In general, hyperhomocysteinemia is increasingly appreciated as a risk factor for various diseases, including osteoporosis. However, its effects in non-adults remain largely unknown. Our aim was to determine whether dietary-caused increased homocysteine levels have deleterious effects on bone structure during growth. Main methods: We developed a model of moderate hyperhomocysteinemia caused by short-term methionine nutritional overload in growing rats. 30-days-old male Wistar albino rats were randomly assigned to either experimental group subject to a 30-days hypermethionine diet or control group. High-resolution 3D assessment of bone geometry and microarchitecture, as well as fluorescence spectroscopic analysis of bone matrix were performed. Key findings: Short-term moderate hyperhomocysteinemia (similar to 30 mu mol/L) achieved in the study notably affected bone and cartilage characteristics. Parameters of the cortical bone geometry in the experimental group indicated peculiar reorganization of the bone cross-section. Trabecular bone microarchitecture was especially sensitive to hyperhomocysteinemia showing clearly negative bone balance in the experimental group (almost 30% reduced bone volume, mainly due to similar to 25% decrease in trabecular number as well as markedly reduced trabecular connections). Fluorescent spectroscopy of bone matrix revealed multiple alterations to collagen spectra due to homocysteine accumulation in bone, indicative of broken collagenous cross-links. Significance: Given that appropriate accrual of bone mass during growth has important effects on the risk of osteoporosis in adulthood, understanding the skeletal effects of dietary-induced hyperhomocysteinemia in nonadults is essential for interpreting its importance as a modifiable risk factor for osteoporosis and improving programs to preserve/re-establish bone health.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Life Sciences", title = "Moderate hyperhomocysteinemia induced by short-term dietary methionine overload alters bone microarchitecture and collagen features during growth", pages = "16-9", volume = "191", doi = "10.1016/j.lfs.2017.10.008" }
Milovanović, P., Hrncic, D., Radotić, K., Stanković, M., Mutavdžić, D., Djonic, D., Rasic-Marković, A., Djuric, D., Stanojlović, O.,& Djuric, M.. (2017). Moderate hyperhomocysteinemia induced by short-term dietary methionine overload alters bone microarchitecture and collagen features during growth. in Life Sciences Pergamon-Elsevier Science Ltd, Oxford., 191, 9-16. https://doi.org/10.1016/j.lfs.2017.10.008
Milovanović P, Hrncic D, Radotić K, Stanković M, Mutavdžić D, Djonic D, Rasic-Marković A, Djuric D, Stanojlović O, Djuric M. Moderate hyperhomocysteinemia induced by short-term dietary methionine overload alters bone microarchitecture and collagen features during growth. in Life Sciences. 2017;191:9-16. doi:10.1016/j.lfs.2017.10.008 .
Milovanović, Petar, Hrncic, Dragan, Radotić, Ksenija, Stanković, Mira, Mutavdžić, Dragosav, Djonic, Danijela, Rasic-Marković, Aleksandra, Djuric, Dragan, Stanojlović, Olivera, Djuric, Marija, "Moderate hyperhomocysteinemia induced by short-term dietary methionine overload alters bone microarchitecture and collagen features during growth" in Life Sciences, 191 (2017):9-16, https://doi.org/10.1016/j.lfs.2017.10.008 . .