Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study
Само за регистроване кориснике
2016
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to... cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap).
Кључне речи:
Xylem sap / Transition metals / Monosilicic acid / EPR / Cucumius sativus LИзвор:
Biometals, 2016, 29, 5, 945-951Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Минерални стрес и адаптације биљака на маргиналним пољопривредним земљиштима (RS-173028)
- Биомаркери у неуродегенеративним и малигним процесима (RS-41005)
DOI: 10.1007/s10534-016-9966-9
ISSN: 0966-0844
PubMed: 27502949
WoS: 000385252400017
Scopus: 2-s2.0-84981163477
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Stević, Nenad AU - Korać Jačić, Jelena AU - Pavlović, Jelena AU - Nikolic, Miroslav PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1008 AB - The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap). PB - Springer, Dordrecht T2 - Biometals T1 - Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study EP - 951 IS - 5 SP - 945 VL - 29 DO - 10.1007/s10534-016-9966-9 ER -
@article{ author = "Stević, Nenad and Korać Jačić, Jelena and Pavlović, Jelena and Nikolic, Miroslav", year = "2016", abstract = "The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap).", publisher = "Springer, Dordrecht", journal = "Biometals", title = "Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study", pages = "951-945", number = "5", volume = "29", doi = "10.1007/s10534-016-9966-9" }
Stević, N., Korać Jačić, J., Pavlović, J.,& Nikolic, M.. (2016). Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study. in Biometals Springer, Dordrecht., 29(5), 945-951. https://doi.org/10.1007/s10534-016-9966-9
Stević N, Korać Jačić J, Pavlović J, Nikolic M. Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study. in Biometals. 2016;29(5):945-951. doi:10.1007/s10534-016-9966-9 .
Stević, Nenad, Korać Jačić, Jelena, Pavlović, Jelena, Nikolic, Miroslav, "Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study" in Biometals, 29, no. 5 (2016):945-951, https://doi.org/10.1007/s10534-016-9966-9 . .