TY  - JOUR
AU  - Pejin, Boris
AU  - Simonović, Mladen
AU  - Talevska, Aleksandra
AU  - Glumac, Miodrag
AU  - Jakimov, Dimitar
AU  - Kojić, Vesna
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1511
AB  - The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A neglected natural source for targeting glioblastoma
EP  - 1860
IS  - 11
SP  - 1856
VL  - 35
DO  - 10.1080/14786419.2019.1638386
ER  - 

@article{
author = "Pejin, Boris and Simonović, Mladen and Talevska, Aleksandra and Glumac, Miodrag and Jakimov, Dimitar and Kojić, Vesna",
year = "2021",
abstract = "The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A neglected natural source for targeting glioblastoma",
pages = "1860-1856",
number = "11",
volume = "35",
doi = "10.1080/14786419.2019.1638386"
}

Pejin, B., Simonović, M., Talevska, A., Glumac, M., Jakimov, D.,& Kojić, V.. (2021). A neglected natural source for targeting glioblastoma. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 35(11), 1856-1860.
https://doi.org/10.1080/14786419.2019.1638386

Pejin B, Simonović M, Talevska A, Glumac M, Jakimov D, Kojić V. A neglected natural source for targeting glioblastoma. in Natural Product Research. 2021;35(11):1856-1860.
doi:10.1080/14786419.2019.1638386 .

Pejin, Boris, Simonović, Mladen, Talevska, Aleksandra, Glumac, Miodrag, Jakimov, Dimitar, Kojić, Vesna, "A neglected natural source for targeting glioblastoma" in Natural Product Research, 35, no. 11 (2021):1856-1860,
https://doi.org/10.1080/14786419.2019.1638386 . .

TY  - JOUR
AU  - Talevska, Aleksandra
AU  - Pejin, Boris
AU  - Kojić, Vesna
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1149
AB  - In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 +/- 0.21 mu g/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 +/- 0.13 mu g/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50 values 3.68 +/- 0.19 and 3.14 +/- 0.09 mu g/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A contribution to pharmaceutical biology of freshwater sponges
EP  - 571
IS  - 5
SP  - 568
VL  - 32
DO  - 10.1080/14786419.2017.1315719
ER  - 

@article{
author = "Talevska, Aleksandra and Pejin, Boris and Kojić, Vesna and Berić, Tanja and Stanković, Slaviša",
year = "2018",
abstract = "In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 +/- 0.21 mu g/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 +/- 0.13 mu g/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50 values 3.68 +/- 0.19 and 3.14 +/- 0.09 mu g/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A contribution to pharmaceutical biology of freshwater sponges",
pages = "571-568",
number = "5",
volume = "32",
doi = "10.1080/14786419.2017.1315719"
}

Talevska, A., Pejin, B., Kojić, V., Berić, T.,& Stanković, S.. (2018). A contribution to pharmaceutical biology of freshwater sponges. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 32(5), 568-571.
https://doi.org/10.1080/14786419.2017.1315719

Talevska A, Pejin B, Kojić V, Berić T, Stanković S. A contribution to pharmaceutical biology of freshwater sponges. in Natural Product Research. 2018;32(5):568-571.
doi:10.1080/14786419.2017.1315719 .

Talevska, Aleksandra, Pejin, Boris, Kojić, Vesna, Berić, Tanja, Stanković, Slaviša, "A contribution to pharmaceutical biology of freshwater sponges" in Natural Product Research, 32, no. 5 (2018):568-571,
https://doi.org/10.1080/14786419.2017.1315719 . .