TY  - JOUR
AU  - Andjus, Stefan
AU  - Nikolic, Nadja
AU  - Dobricic, Valerija
AU  - Marjanović, An
AU  - Gačić, Zoran
AU  - Branković, Goran
AU  - Aković, Maja R.
AU  - Paunović, Momir
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1134
AB  - Sponges in the large rivers within the Danube River Basin (DRB) have not been adequately studied. Hence. the aim of this work was to undertake an investigation on the distribution of sponge species in the Danube and Sava rivers. Out of SS localities covered by the study, sponges were found at 25 sites only (46 samples in total). By using morpholotical (light and scanning electron microscopy) and genetic (28S rDNA sequencing) analysis. four species were determined: Ephydatia fluviaulis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Eunapius fragilis (Leidy, 1851), Trachospangilla horrida Weltner, 1893. In the Danube.. the predominant species was found to be E. fluviatilis making approximately 80% of collected samples, while in the Sava River S. lacustris dominated, representing 46% of the river sponges. Our work represents one of the few studies on freshwater sponges within the DRB from long stretches of the large lowland rivers (more than 2500 km of the Danube River and about 900 km of the Sava River). Moreover, molecular analysis for the identification of freshwater sponges was applied on the material collected from a wide area. thus contributing to the systematic studies on the distribution and abundance of the European freshwater invertebrate fauna in general.
PB  - Pagepress Publ, Pavia
T2  - Journal of Limnology
T1  - Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study
EP  - 208
IS  - 2
SP  - 199
VL  - 77
DO  - 10.4081/jlimnol.2017.1677
ER  - 

@article{
author = "Andjus, Stefan and Nikolic, Nadja and Dobricic, Valerija and Marjanović, An and Gačić, Zoran and Branković, Goran and Aković, Maja R. and Paunović, Momir",
year = "2018",
abstract = "Sponges in the large rivers within the Danube River Basin (DRB) have not been adequately studied. Hence. the aim of this work was to undertake an investigation on the distribution of sponge species in the Danube and Sava rivers. Out of SS localities covered by the study, sponges were found at 25 sites only (46 samples in total). By using morpholotical (light and scanning electron microscopy) and genetic (28S rDNA sequencing) analysis. four species were determined: Ephydatia fluviaulis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Eunapius fragilis (Leidy, 1851), Trachospangilla horrida Weltner, 1893. In the Danube.. the predominant species was found to be E. fluviatilis making approximately 80% of collected samples, while in the Sava River S. lacustris dominated, representing 46% of the river sponges. Our work represents one of the few studies on freshwater sponges within the DRB from long stretches of the large lowland rivers (more than 2500 km of the Danube River and about 900 km of the Sava River). Moreover, molecular analysis for the identification of freshwater sponges was applied on the material collected from a wide area. thus contributing to the systematic studies on the distribution and abundance of the European freshwater invertebrate fauna in general.",
publisher = "Pagepress Publ, Pavia",
journal = "Journal of Limnology",
title = "Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study",
pages = "208-199",
number = "2",
volume = "77",
doi = "10.4081/jlimnol.2017.1677"
}

Andjus, S., Nikolic, N., Dobricic, V., Marjanović, A., Gačić, Z., Branković, G., Aković, M. R.,& Paunović, M.. (2018). Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study. in Journal of Limnology
Pagepress Publ, Pavia., 77(2), 199-208.
https://doi.org/10.4081/jlimnol.2017.1677

Andjus S, Nikolic N, Dobricic V, Marjanović A, Gačić Z, Branković G, Aković MR, Paunović M. Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study. in Journal of Limnology. 2018;77(2):199-208.
doi:10.4081/jlimnol.2017.1677 .

Andjus, Stefan, Nikolic, Nadja, Dobricic, Valerija, Marjanović, An, Gačić, Zoran, Branković, Goran, Aković, Maja R., Paunović, Momir, "Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study" in Journal of Limnology, 77, no. 2 (2018):199-208,
https://doi.org/10.4081/jlimnol.2017.1677 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Simonovic, Predrag
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1913
AB  - One of the major limitations in performing ecogenotoxicological studies is the distance between research field and the laboratory. As some of the methods used in ecogenotoxicology require fresh biological material with intact cell viability, transfer of samples to the laboratory within a few hours after sampling is usually required. To overcome this issue, we have introduced cryopreservation in our research as a possible solution. Cryopreservation is a method which includes preservation of intact, living cells at low temperature for a long time. In natural conditions freezing, forming of ice crystals and dehydration could destroy cell structures. To avoid this consequence, specific compounds were introduced, cryoprotective agents, in the method of cryopreservation. The main characteristic of these compounds is their ability to reduce ice crystal formation in cells at any temperature.
We have applied cryopreservation in the evaluation of genotoxic potential along different river streams (the Adige River, the Sava River and the Velika Morava River basin). For this purpose, we focused on the level of DNA damage of cryopreserved fish blood cells (Salmo cenerinus, Salmo marmoratus, Alburnus alburnus) by using the comet assay.
To test whether cryopreservation has the impact on cell viability, or that it induces additional DNA damage, we employed preliminary experiments in 4 Abramis brama and 8 A. alburnus specimens. Namely, from every specimen two blood samples were taken: one for analyzing cells viability and the level of DNA damage of fresh blood, and another for observing cell viability and DNA damage of cryopreserved samples. The viability of cell blood was determined by using acridine orange/ethidium bromide differential staining. For analyzing the level of DNA damage alkaline comet assay was used. Obtained results indicated that cryopreserved blood cells had approximately the same viability and the level of DNA damage as nonpreserved blood samples.
According to our results, cryopreservation is a very useful method in genotoxicology and could have many benefits: blood samples should not be analyzed immediately after sampling; samples could be transported in liquid nitrogen without concern about additional DNA damage.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1913
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir and Simonovic, Predrag and Vuković-Gačić, Branka",
year = "2018",
abstract = "One of the major limitations in performing ecogenotoxicological studies is the distance between research field and the laboratory. As some of the methods used in ecogenotoxicology require fresh biological material with intact cell viability, transfer of samples to the laboratory within a few hours after sampling is usually required. To overcome this issue, we have introduced cryopreservation in our research as a possible solution. Cryopreservation is a method which includes preservation of intact, living cells at low temperature for a long time. In natural conditions freezing, forming of ice crystals and dehydration could destroy cell structures. To avoid this consequence, specific compounds were introduced, cryoprotective agents, in the method of cryopreservation. The main characteristic of these compounds is their ability to reduce ice crystal formation in cells at any temperature.
We have applied cryopreservation in the evaluation of genotoxic potential along different river streams (the Adige River, the Sava River and the Velika Morava River basin). For this purpose, we focused on the level of DNA damage of cryopreserved fish blood cells (Salmo cenerinus, Salmo marmoratus, Alburnus alburnus) by using the comet assay.
To test whether cryopreservation has the impact on cell viability, or that it induces additional DNA damage, we employed preliminary experiments in 4 Abramis brama and 8 A. alburnus specimens. Namely, from every specimen two blood samples were taken: one for analyzing cells viability and the level of DNA damage of fresh blood, and another for observing cell viability and DNA damage of cryopreserved samples. The viability of cell blood was determined by using acridine orange/ethidium bromide differential staining. For analyzing the level of DNA damage alkaline comet assay was used. Obtained results indicated that cryopreserved blood cells had approximately the same viability and the level of DNA damage as nonpreserved blood samples.
According to our results, cryopreservation is a very useful method in genotoxicology and could have many benefits: blood samples should not be analyzed immediately after sampling; samples could be transported in liquid nitrogen without concern about additional DNA damage.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1913"
}

Jovanović Marić, J., Đorđević Aleksić, J., Kolarević, S., Kračun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Paunović, M., Simonovic, P.,& Vuković-Gačić, B.. (2018). Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1913

Jovanović Marić J, Đorđević Aleksić J, Kolarević S, Kračun-Kolarević M, Kostić-Vuković J, Sunjog K, Paunović M, Simonovic P, Vuković-Gačić B. Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1913 .

Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, Simonovic, Predrag, Vuković-Gačić, Branka, "Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1913 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jovanović, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Piria, Marina
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1160
AB  - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .
PB  - Springer International Publishing Ag, Cham
T2  - International Journal of Environmental Research
T1  - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
EP  - 712
IS  - 5
SP  - 703
VL  - 12
DO  - 10.1007/s41742-018-0127-6
ER  - 

@article{
author = "Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simic, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .",
publisher = "Springer International Publishing Ag, Cham",
journal = "International Journal of Environmental Research",
title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study",
pages = "712-703",
number = "5",
volume = "12",
doi = "10.1007/s41742-018-0127-6"
}

Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simic, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vukovic-Gacic, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research
Springer International Publishing Ag, Cham., 12(5), 703-712.
https://doi.org/10.1007/s41742-018-0127-6

Kolarević S, Sunjog K, Kracun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simic V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vukovic-Gacic B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;12(5):703-712.
doi:10.1007/s41742-018-0127-6 .

Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simic, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vukovic-Gacic, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research, 12, no. 5 (2018):703-712,
https://doi.org/10.1007/s41742-018-0127-6 . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1060
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Milosković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1007
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Simonović, Predrag and Simic, Vladica and Milosković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450"
}

Kolarević, S., Aborgiba, M., Kracun-Kolarević, M., Kostić-Vuković, J., Simonović, P., Simic, V., Milosković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kracun-Kolarević M, Kostić-Vuković J, Simonović P, Simic V, Milosković A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Simonović, Predrag, Simic, Vladica, Milosković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .

TY  - JOUR
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, Jovana
AU  - Marković, Vanja
AU  - Illic, Marija
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Gačić, Zoran
AU  - Diamantini, Elena
AU  - Stella, Elisa
AU  - Petrović, Mira
AU  - Majone, Bruno
AU  - Bellin, Alberto
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/977
AB  - In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin
EP  - 1391
SP  - 1383
VL  - 571
DO  - 10.1016/j.scitotenv.2016.07.099
ER  - 

@article{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, Jovana and Marković, Vanja and Illic, Marija and Simonović, Predrag and Simic, Vladica and Gačić, Zoran and Diamantini, Elena and Stella, Elisa and Petrović, Mira and Majone, Bruno and Bellin, Alberto and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin",
pages = "1391-1383",
volume = "571",
doi = "10.1016/j.scitotenv.2016.07.099"
}

Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Marković, V., Illic, M., Simonović, P., Simic, V., Gačić, Z., Diamantini, E., Stella, E., Petrović, M., Majone, B., Bellin, A., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 571, 1383-1391.
https://doi.org/10.1016/j.scitotenv.2016.07.099

Kracun-Kolarević M, Kolarević S, Jovanović J, Marković V, Illic M, Simonović P, Simic V, Gačić Z, Diamantini E, Stella E, Petrović M, Majone B, Bellin A, Paunović M, Vukovic-Gacic B. Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment. 2016;571:1383-1391.
doi:10.1016/j.scitotenv.2016.07.099 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, Jovana, Marković, Vanja, Illic, Marija, Simonović, Predrag, Simic, Vladica, Gačić, Zoran, Diamantini, Elena, Stella, Elisa, Petrović, Mira, Majone, Bruno, Bellin, Alberto, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin" in Science of the Total Environment, 571 (2016):1383-1391,
https://doi.org/10.1016/j.scitotenv.2016.07.099 . .

TY  - JOUR
AU  - Vrzel, Janja
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Ogrinc, Nives
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1016
AB  - Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin
EP  - 1471
SP  - 1460
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.213
ER  - 

@article{
author = "Vrzel, Janja and Vukovic-Gacic, Branka and Kolarević, Stoimir and Gačić, Zoran and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Ogrinc, Nives",
year = "2016",
abstract = "Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin",
pages = "1471-1460",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.213"
}

Vrzel, J., Vukovic-Gacic, B., Kolarević, S., Gačić, Z., Kracun-Kolarević, M., Kostić-Vuković, J., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Ogrinc, N.. (2016). Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1460-1471.
https://doi.org/10.1016/j.scitotenv.2016.07.213

Vrzel J, Vukovic-Gacic B, Kolarević S, Gačić Z, Kracun-Kolarević M, Kostić-Vuković J, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Ogrinc N. Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment. 2016;573:1460-1471.
doi:10.1016/j.scitotenv.2016.07.213 .

Vrzel, Janja, Vukovic-Gacic, Branka, Kolarević, Stoimir, Gačić, Zoran, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Ogrinc, Nives, "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin" in Science of the Total Environment, 573 (2016):1460-1471,
https://doi.org/10.1016/j.scitotenv.2016.07.213 . .

TY  - JOUR
AU  - Aborgiba, Mustafa
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Elbahi, Samia
AU  - Knezevic-Vukcević, Jelena
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/936
AB  - Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River
EP  - 367
SP  - 358
VL  - 540
DO  - 10.1016/j.scitotenv.2015.03.120
ER  - 

@article{
author = "Aborgiba, Mustafa and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Elbahi, Samia and Knezevic-Vukcević, Jelena and Lenhardt, Mirjana and Paunović, Momir and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2016",
abstract = "Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River",
pages = "367-358",
volume = "540",
doi = "10.1016/j.scitotenv.2015.03.120"
}

Aborgiba, M., Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Elbahi, S., Knezevic-Vukcević, J., Lenhardt, M., Paunović, M., Gačić, Z.,& Vukovic-Gacic, B.. (2016). Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment
Elsevier, Amsterdam., 540, 358-367.
https://doi.org/10.1016/j.scitotenv.2015.03.120

Aborgiba M, Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Elbahi S, Knezevic-Vukcević J, Lenhardt M, Paunović M, Gačić Z, Vukovic-Gacic B. Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment. 2016;540:358-367.
doi:10.1016/j.scitotenv.2015.03.120 .

Aborgiba, Mustafa, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Elbahi, Samia, Knezevic-Vukcević, Jelena, Lenhardt, Mirjana, Paunović, Momir, Gačić, Zoran, Vukovic-Gacic, Branka, "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River" in Science of the Total Environment, 540 (2016):358-367,
https://doi.org/10.1016/j.scitotenv.2015.03.120 . .

TY  - JOUR
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Atanacković, Ana
AU  - Marković, Vanja
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/925
AB  - Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl2) was evaluated in Limnodrilus udekemianus, cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 mu M), ET (0.004. 0.04, 0.4 and 4 mu M) and CdCl2 (0.004, 0.04, 0.4, 4 and 40 mu M). Mortality of worms was observed only for CdCl2 (4 and 40 mu M). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 mu M), ET (4 mu M) and CdCl2 (0.4 and 4 mu M). All tested substances induced significant increase of DNA damage except 0.004 mu M of ET. L. udekemianus being sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 mu M induced DNA damage is similar to ones detected in wastewaters.
PB  - Springer International Publishing Ag, Cham
T2  - Water Air and Soil Pollution
T1  - Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay
IS  - 8
VL  - 226
DO  - 10.1007/s11270-015-2511-6
ER  - 

@article{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Atanacković, Ana and Marković, Vanja and Gačić, Zoran and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2015",
abstract = "Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl2) was evaluated in Limnodrilus udekemianus, cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 mu M), ET (0.004. 0.04, 0.4 and 4 mu M) and CdCl2 (0.004, 0.04, 0.4, 4 and 40 mu M). Mortality of worms was observed only for CdCl2 (4 and 40 mu M). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 mu M), ET (4 mu M) and CdCl2 (0.4 and 4 mu M). All tested substances induced significant increase of DNA damage except 0.004 mu M of ET. L. udekemianus being sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 mu M induced DNA damage is similar to ones detected in wastewaters.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Water Air and Soil Pollution",
title = "Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay",
number = "8",
volume = "226",
doi = "10.1007/s11270-015-2511-6"
}

Kracun-Kolarević, M., Kolarević, S., Atanacković, A., Marković, V., Gačić, Z., Paunović, M.,& Vukovic-Gacic, B.. (2015). Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay. in Water Air and Soil Pollution
Springer International Publishing Ag, Cham., 226(8).
https://doi.org/10.1007/s11270-015-2511-6

Kracun-Kolarević M, Kolarević S, Atanacković A, Marković V, Gačić Z, Paunović M, Vukovic-Gacic B. Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay. in Water Air and Soil Pollution. 2015;226(8).
doi:10.1007/s11270-015-2511-6 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Atanacković, Ana, Marković, Vanja, Gačić, Zoran, Paunović, Momir, Vukovic-Gacic, Branka, "Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay" in Water Air and Soil Pollution, 226, no. 8 (2015),
https://doi.org/10.1007/s11270-015-2511-6 . .