TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1014
AB  - The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.
PB  - Wiley, Hoboken
T2  - Clean-Soil Air Water
T1  - Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus
EP  - 1476
IS  - 11
SP  - 1471
VL  - 44
DO  - 10.1002/clen.201500482
ER  - 

@article{
author = "Kolarević, Stoimir and Gačić, Zoran and Kostić-Vuković, Jovana and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.",
publisher = "Wiley, Hoboken",
journal = "Clean-Soil Air Water",
title = "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus",
pages = "1476-1471",
number = "11",
volume = "44",
doi = "10.1002/clen.201500482"
}

Kolarević, S., Gačić, Z., Kostić-Vuković, J., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water
Wiley, Hoboken., 44(11), 1471-1476.
https://doi.org/10.1002/clen.201500482

Kolarević S, Gačić Z, Kostić-Vuković J, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water. 2016;44(11):1471-1476.
doi:10.1002/clen.201500482 .

Kolarević, Stoimir, Gačić, Zoran, Kostić-Vuković, Jovana, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus" in Clean-Soil Air Water, 44, no. 11 (2016):1471-1476,
https://doi.org/10.1002/clen.201500482 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/822
AB  - The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 mu M) with 0.4 mu M being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 mu M 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.
PB  - Elsevier Sci Ltd, Oxford
T2  - Environmental Pollution
T1  - The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus
EP  - 150
SP  - 145
VL  - 191
DO  - 10.1016/j.envpol.2014.04.024
ER  - 

@article{
author = "Gačić, Zoran and Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 mu M) with 0.4 mu M being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 mu M 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Environmental Pollution",
title = "The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus",
pages = "150-145",
volume = "191",
doi = "10.1016/j.envpol.2014.04.024"
}

Gačić, Z., Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2014). The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in Environmental Pollution
Elsevier Sci Ltd, Oxford., 191, 145-150.
https://doi.org/10.1016/j.envpol.2014.04.024

Gačić Z, Kolarević S, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in Environmental Pollution. 2014;191:145-150.
doi:10.1016/j.envpol.2014.04.024 .

Gačić, Zoran, Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus" in Environmental Pollution, 191 (2014):145-150,
https://doi.org/10.1016/j.envpol.2014.04.024 . .

TY  - JOUR
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/820
AB  - Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city's main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.
PB  - Springer, Dordrecht
T2  - Hydrobiologia
T1  - Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress
EP  - 231
IS  - 1
SP  - 221
VL  - 735
DO  - 10.1007/s10750-013-1513-x
ER  - 

@article{
author = "Vukovic-Gacic, Branka and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran",
year = "2014",
abstract = "Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city's main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.",
publisher = "Springer, Dordrecht",
journal = "Hydrobiologia",
title = "Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress",
pages = "231-221",
number = "1",
volume = "735",
doi = "10.1007/s10750-013-1513-x"
}

Vukovic-Gacic, B., Kolarević, S., Sunjog, K., Tomović, J. M., Knezevic-Vukcević, J., Paunović, M.,& Gačić, Z.. (2014). Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. in Hydrobiologia
Springer, Dordrecht., 735(1), 221-231.
https://doi.org/10.1007/s10750-013-1513-x

Vukovic-Gacic B, Kolarević S, Sunjog K, Tomović JM, Knezevic-Vukcević J, Paunović M, Gačić Z. Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. in Hydrobiologia. 2014;735(1):221-231.
doi:10.1007/s10750-013-1513-x .

Vukovic-Gacic, Branka, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, "Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress" in Hydrobiologia, 735, no. 1 (2014):221-231,
https://doi.org/10.1007/s10750-013-1513-x . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Kracun, Margareta
AU  - Vasiljević, Bozica
AU  - Tomović, Jelena M
AU  - Vukovic-Gacic, Branka
AU  - Gačić, Zoran
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/703
AB  - This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay
EP  - 251
IS  - 2
SP  - 243
VL  - 93
DO  - 10.1016/j.chemosphere.2013.04.073
ER  - 

@article{
author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Kracun, Margareta and Vasiljević, Bozica and Tomović, Jelena M and Vukovic-Gacic, Branka and Gačić, Zoran",
year = "2013",
abstract = "This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay",
pages = "251-243",
number = "2",
volume = "93",
doi = "10.1016/j.chemosphere.2013.04.073"
}

Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Kracun, M., Vasiljević, B., Tomović, J. M., Vukovic-Gacic, B.,& Gačić, Z.. (2013). Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 93(2), 243-251.
https://doi.org/10.1016/j.chemosphere.2013.04.073

Kolarević S, Knezevic-Vukcević J, Paunović M, Kracun M, Vasiljević B, Tomović JM, Vukovic-Gacic B, Gačić Z. Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere. 2013;93(2):243-251.
doi:10.1016/j.chemosphere.2013.04.073 .

Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Kracun, Margareta, Vasiljević, Bozica, Tomović, Jelena M, Vukovic-Gacic, Branka, Gačić, Zoran, "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay" in Chemosphere, 93, no. 2 (2013):243-251,
https://doi.org/10.1016/j.chemosphere.2013.04.073 . .