TY  - JOUR
AU  - Nedzved, Aleksander
AU  - Mitrović, Aleksandra Lj.
AU  - Savić, Aleksandar G
AU  - Mutavdžić, Dragosav
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Garab, Gyozo
AU  - Starovoytov, Valery
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1116
AB  - Measurements of various anatomical characteristics of wood cells are of great importance in research of wood structure, either for the evaluation of environmental influences or for estimation of wood quality. We present and test an automatic image processing morphometric method for the analysis of tracheid double wall thickness. A new algorithm of image analysis was developed. It uses morphological processing of structural elements with the different orientations from distance maps to analyze tracheid double wall thickness distribution separately for radial walls, tangential walls, and cell corners. For testing the performance of the method, we used confocal laser scanning microscopy images of stem cross-sections of juvenile Picea omorika trees exposed to long-term static bending. As a response to mechanical stress, conifers form compression wood (CW), which occurs in a range of gradations from near normal wood (NW) to severe CW. However, visual detection of compression wood severity, more precisely the determination of mild CW, is difficult. One of the anatomic features that characterize CW is increased wall thickness. After testing proposed automatic image processing morphometric method for the analysis of tracheid double wall thickness separately for radial walls, tangential walls and cell corners, combined with statistical analysis, we could suggest it as a tool for estimation of compression wood severity, or for estimation and gradation of changes in tracheid cell wall thickness as a response to environmental influences during growth and developmental process.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending
EP  - 1356
IS  - 5
SP  - 1347
VL  - 32
DO  - 10.1007/s00468-018-1716-x
ER  - 

@article{
author = "Nedzved, Aleksander and Mitrović, Aleksandra Lj. and Savić, Aleksandar G and Mutavdžić, Dragosav and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Garab, Gyozo and Starovoytov, Valery and Radotić, Ksenija",
year = "2018",
abstract = "Measurements of various anatomical characteristics of wood cells are of great importance in research of wood structure, either for the evaluation of environmental influences or for estimation of wood quality. We present and test an automatic image processing morphometric method for the analysis of tracheid double wall thickness. A new algorithm of image analysis was developed. It uses morphological processing of structural elements with the different orientations from distance maps to analyze tracheid double wall thickness distribution separately for radial walls, tangential walls, and cell corners. For testing the performance of the method, we used confocal laser scanning microscopy images of stem cross-sections of juvenile Picea omorika trees exposed to long-term static bending. As a response to mechanical stress, conifers form compression wood (CW), which occurs in a range of gradations from near normal wood (NW) to severe CW. However, visual detection of compression wood severity, more precisely the determination of mild CW, is difficult. One of the anatomic features that characterize CW is increased wall thickness. After testing proposed automatic image processing morphometric method for the analysis of tracheid double wall thickness separately for radial walls, tangential walls and cell corners, combined with statistical analysis, we could suggest it as a tool for estimation of compression wood severity, or for estimation and gradation of changes in tracheid cell wall thickness as a response to environmental influences during growth and developmental process.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending",
pages = "1356-1347",
number = "5",
volume = "32",
doi = "10.1007/s00468-018-1716-x"
}

Nedzved, A., Mitrović, A. Lj., Savić, A. G., Mutavdžić, D., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Garab, G., Starovoytov, V.,& Radotić, K.. (2018). Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 32(5), 1347-1356.
https://doi.org/10.1007/s00468-018-1716-x

Nedzved A, Mitrović AL, Savić AG, Mutavdžić D, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Garab G, Starovoytov V, Radotić K. Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending. in Trees-Structure and Function. 2018;32(5):1347-1356.
doi:10.1007/s00468-018-1716-x .

Nedzved, Aleksander, Mitrović, Aleksandra Lj., Savić, Aleksandar G, Mutavdžić, Dragosav, Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Garab, Gyozo, Starovoytov, Valery, Radotić, Ksenija, "Automatic image processing morphometric method for the analysis of tracheid double wall thickness tested on juvenile Picea omorika trees exposed to static bending" in Trees-Structure and Function, 32, no. 5 (2018):1347-1356,
https://doi.org/10.1007/s00468-018-1716-x . .

TY  - JOUR
AU  - Savić, Aleksandar G
AU  - Mitrović, Aleksandra Lj.
AU  - Donaldson, Lloyd
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/989
AB  - Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.
PB  - Cambridge Univ Press, New York
T2  - Microscopy and Microanalysis
T1  - Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity
EP  - 367
IS  - 2
SP  - 361
VL  - 22
DO  - 10.1017/S143192761600009X
ER  - 

@article{
author = "Savić, Aleksandar G and Mitrović, Aleksandra Lj. and Donaldson, Lloyd and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Garab, Gyozo and Radotić, Ksenija",
year = "2016",
abstract = "Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.",
publisher = "Cambridge Univ Press, New York",
journal = "Microscopy and Microanalysis",
title = "Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity",
pages = "367-361",
number = "2",
volume = "22",
doi = "10.1017/S143192761600009X"
}

Savić, A. G., Mitrović, A. Lj., Donaldson, L., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Garab, G.,& Radotić, K.. (2016). Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity. in Microscopy and Microanalysis
Cambridge Univ Press, New York., 22(2), 361-367.
https://doi.org/10.1017/S143192761600009X

Savić AG, Mitrović AL, Donaldson L, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Garab G, Radotić K. Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity. in Microscopy and Microanalysis. 2016;22(2):361-367.
doi:10.1017/S143192761600009X .

Savić, Aleksandar G, Mitrović, Aleksandra Lj., Donaldson, Lloyd, Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Garab, Gyozo, Radotić, Ksenija, "Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity" in Microscopy and Microanalysis, 22, no. 2 (2016):361-367,
https://doi.org/10.1017/S143192761600009X . .